R9-FS2
(Plasmid
#125209)
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PurposeExpressing human Rad9b frameshift mutation found in a neural tube defect patient
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125209 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA-3.1-N-EGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRad9b
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SpeciesH. sapiens (human)
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Mutationp.A401Rfs4X
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Entrez GeneRAD9B
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
R9-FS2 was a gift from Richard Finnell (Addgene plasmid # 125209 ; http://n2t.net/addgene:125209 ; RRID:Addgene_125209) -
For your References section:
Loss of RAD9B impairs early neural development and contributes to the risk for human spina bifida. Cao X, Tian T, Steele JW, Cabrera RM, Aguiar-Pulido V, Wadhwa S, Bhavani N, Bi P, Gargurevich NH, Hoffman EN, Cai CQ, Marini NJ, Yang W, Shaw GM, Ross ME, Finnell RH, Lei Y. Hum Mutat. 2020 Apr;41(4):786-799. doi: 10.1002/humu.23969. Epub 2020 Jan 16. 10.1002/humu.23969 PubMed 31898828
