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Plasmid#110612PurposeBTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoterDepositorInsertNanoluc
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-Neo-FF3
Plasmid#11665Purpose3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of Neomycin and miR30-based shRNA targeting firefly luciferaseDepositorInsertFF3
UseLentiviralTagsNeoExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2(T309A)
Plasmid#86625PurposeExpresses eGFP-tagged human Akt2(T309A) phosphomutantDepositorInsertAkt2(T309A) (AKT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Luc-Sox2-3'UTR
Plasmid#63895PurposeLuciferase reporter of mouse wildtype Sox2 3'UTRDepositorInsertSRY (sex determining region Y)-box 2 (Sox2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter CREB site mutant pGL3Basic
Plasmid#32732DepositorInsertCCND1 Promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-51 CREB site TAACGTCA deletionPromoter-962 CCND1 PromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL3
Plasmid#169108PurposeExpresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NRASlong
Plasmid#13797DepositorInsertNRAS (NRAS Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRenilla (GB0056)
Plasmid#68198PurposeProvides the CDS of the Renilla protein as a level 0 GoldenBraid partDepositorInsertRenilla coding region
UseLuciferase and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJTX-GL3
Plasmid#11981PurposeReporter gene with mouse c-jun promoter and point mutation of AP1 binding siteDepositorInsertc-jun promoter (Jun Mouse)
UseLuciferaseTagsLucExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc
Plasmid#200123PurposeRetroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTS-hFluc-HA-GFP11
Plasmid#177726PurposeFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
UseTagsGFP11, HA, and Mitochondrial Targeting SequenceExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJSTX-GL3
Plasmid#11982PurposeReporter gene with mouse c-jun promoter and point mutation of MEF2 and AP1 binding sitesDepositorInsertc-jun promoter (Jun Mouse)
UseLuciferaseTagsLucExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRF+275Dux4
Plasmid#21293DepositorInsertDUX 4 (DUX4 DUX 4, Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJSX-GL3
Plasmid#11980PurposeReporter gene with mouse c-jun promoter and point mutation of MEF2 binding siteDepositorInsertc-jun promoter (Jun Mouse)
UseLuciferaseTagsLucExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-R9-GFP-HiBit
Plasmid#170990PurposeFor bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-R9-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 4xSBR1
Plasmid#15706DepositorInsertp15 4xSBR1 (CDKN2B Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 4xSBR2
Plasmid#15709DepositorInsertp15 4xSBR2 (CDKN2B Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-Smad4-1719
Plasmid#49383PurposeInsert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.DepositorInsertSmad family member 4 (SMAD4 Mouse, Human)
UseLuciferaseTagsExpressionMutationPromoterHuman phosphoglycerate kinase (PGK) promoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1) site mutant pGL3Basic
Plasmid#32733DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) site CTTTGAT deletionPromoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL2
Plasmid#169107PurposeExpresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only