Addgene

We narrowed to 14,719 results for: LEA

Showing: 3281 - 3300 of 14719 results

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pGEXndoE(E662Q)

Plasmid

#47716

Purpose

Expresses GST-EndoE(E662Q) from E. faecalis in E. coli

Depositor

Vincent Fischetti, Mattias Collin

Article

Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

Insert

ndoE

Use

Tags

GST-EndoE(E186Q) and Glutathione S-transferase

Expression

Bacterial

Mutation

Changed Glu-662 to Gln

Promoter

tac

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRS423-SgH

Plasmid

#163972

Purpose

Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-NT

Plasmid

#164857

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-NT

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pPS832

Plasmid

#1357

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
Hy_pPepck1 eGFP SV40

Plasmid

#132644

Purpose

Expresses eGFP mRNA from the Drosophila Pepck1 promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Pepck1

Available since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-NT

Plasmid

#164861

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-NT

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-1

Plasmid

#164858

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NEO-CAG-BFP-pA-GFP-pA

Plasmid

#149344

Purpose

Vector for targeting the Replace reporter to the AAVS1 locus

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-HIRAmut-polyA

Plasmid

#59782

Purpose

This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Use

Tags

Expression

Mammalian

Mutation

Change from 'GATCGAAGTTTG' to 'GAc…

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF10

Plasmid

#13109

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF10

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pTEI134

Plasmid

#126071

Purpose

Vector for expression of plastid targeted YFP1-10 (T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

YFP1-10

Expression

Plant

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0007

Plasmid

#117534

Purpose

Level0 Golden Gate part: CDS, LbCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

LbCas12a (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF5

Plasmid

#13104

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF5

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-CBh-Cas9n-pA

Plasmid

#82617

Purpose

Expresses Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

SpCas9n

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA nLuc SV40

Plasmid

#132654

Purpose

Expresses nanoLuciferase (nLuc) mRNA from the Drosophila Metallothionein A promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

MtnA

Available since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLY103

Plasmid

#130937

Purpose

Engineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Plux2

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF64

Plasmid

#13200

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF64

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF63

Plasmid

#13199

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF63

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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HS-H2B-RFP

Plasmid

#105953

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

H2B

Use

Tags

RFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0021

Plasmid

#117532

Purpose

Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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