We narrowed to 7,030 results for: 11

1. p55-H2B-Dendra2

   Plasmid

   #80609

   Purpose

   Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

   Insert

   H2B-Dendra2 (H2BC21 Synthetic, Human)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cx26-msfGFP

   Plasmid

   #69016

   Purpose

   Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Cx26 (Gjb2 Rat)
   

   Use

   Tags

   V206K monomerized super folder GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP C1 SADBs

   Plasmid

   #67154

   Purpose

   pEGFP containing N-terminally EGFP Tagged mouse SADBs

   Depositor

   Ana Carrera, Maria Alvarado-Kristensson

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   Brsk1 (Brsk1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3_V5-APEX2-Sec61B

   Plasmid

   #83412

   Purpose

   Expressed N-term tagged APEX2 on Sec61B in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   V5-APEX2-Sec61B (SEC61B Synthetic, Human)
   

   Use

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FL tension sensor module

   Plasmid

   #101170

   Purpose

   Expresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.

   Depositor

   Carsten Grashoff

   Article

   Ringer et al Nat Methods. 2017 Nov;14(11):1090-1096.

   Insert

   YPet(short)-FL-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPBbsr2-5102HRasCT(Picchu)

   Plasmid

   #209917

   Purpose

   A FRET biosensor for tyrosine kinase activity

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   5102HRasCT
   

   Use

   Tags

   YPet and nTurquoise-GL

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

   Plasmid

   #211687

   Purpose

   U6-sgRNA-EFS-Cas9-P2A-Puro

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   SpyCas9 and guide RNA recipient vector
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CFP-CD82

   Plasmid

   #1818

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   CD82 (CD82 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHis6-hDF-deltaTM-pCMV-9

   Plasmid

   #72587

   Purpose

   human dysferlin no transmembrane domain tagged with his tag

   Depositor

   Renzhi Han

   Article

   Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

   Insert

   dysferlin (DYSF Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   transmembrane domain deleted

   Promoter

   CMV

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

10. shCDK4/6(1)

    Plasmid

    #73554

    Purpose

    vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4 + shRNA against CDK6
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

    Plasmid

    #174892

    Purpose

    amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-Neo-GFP-hMyo19 iso2

    Plasmid

    #210333

    Purpose

    Overexpression of hMyo19

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    hMyo19 (MYO19 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-CRY2(D387A)-mCherry

    Plasmid

    #58369

    Purpose

    Light insensitive CRY2 mutant

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (D387A) (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D387A

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWP1_mHAL

    Plasmid

    #102318

    Purpose

    expression of mouse cDNA of HAL refractory to the guide in plasmid 4

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    Hal (Hal Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-Keap1-Tev-Halo

    Plasmid

    #87545

    Purpose

    expresses C-terminal Halo-tagged human Keap1 in mammalian cells

    Depositor

    Yimon Aye

    Article

    Parvez et al Nat Protoc. 2016 Dec;11(12):2328-2356. d

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    Halo and His-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENN.AAV.CB7.CI.CNDP1-flag.WPRE.rBG

    Plasmid

    #132683

    Purpose

    AAV plasmid encoding mouse CNDP1 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Carnosine dipeptidase 1 (Cndp1 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Chicken b-actin

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQCXIP TBRII

    Plasmid

    #19147

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    Transforming growth factor, beta receptor II (TGFBR2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

18. shCDK4/6(2)

    Plasmid

    #73555

    Purpose

    vector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4 + shRNA against CDK6
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA3 Flag MKK3b(Glu)

    Plasmid

    #50449

    Purpose

    activates p38 in the signaling pathway

    Depositor

    Roger Davis

    Article

    Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

    Insert

    MKK3b (MAP2K3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Serine 218 to Glutamic Acid, changed Thre…

    Promoter

    CMV

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

20. PVT1-luciferase

    Plasmid

    #113350

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    PVT1-enhancer (PVT1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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