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pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

Plasmid

#139999

Purpose

CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP

Use

CRISPR

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

Promoter

CAG

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pWPI-SLC7A11-HA

Plasmid

#201643

Purpose

Over-expression of HA-tagged human SLC7A11

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SLC7A11 (SLC7A11 Human)

Use

Lentiviral

Tags

HA

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE59

Plasmid

#90404

Purpose

STAT3 - Sis-inducible element (SIE) gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

STAT3

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mito-TagRFP-T

Plasmid

#174543

Purpose

labels mitochondria with TagRFP-T

Depositor

Minna Roh-Johnson

Article

Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

Insert

MTS-tagRFP-T

Use

Lentiviral

Tags

tagRFP-T

Expression

Mammalian

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.PI.EGFP.WPRE.bGH

Plasmid

#105530

Purpose

AAV expression of EGFP from CMV promoter

Depositor

James M. Wilson

Has Service

AAV PHP.eB, AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean N1

Plasmid

#27795

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

mCerulean N1

Expression

Mammalian

Available Since

April 19, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-SpCas9

Plasmid

#104588

Purpose

AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

Depositor

Ryohei Yasuda

Article

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Has Service

AAV8

Insert

Streptococcus pyogenes Cas9

Use

AAV and CRISPR

Tags

Myc

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#209723

Purpose

Expresses mitochondrially targeted low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(mito).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-DIO-NaChBac-dTomato

Plasmid

#236252

Purpose

Cre dependent NaChBac-TdTomato expression

Depositor

Janelia Viral Tools

Article

Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

Insert

NaChBac-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Promoter

hSyn

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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PEmax_2star_pet21A

Plasmid

#227675

Purpose

Expression plasmid for PEMAX** protein in bacteria.

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

PEmax_2_star

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-ChrimsonR-tdT

Plasmid

#59169

Purpose

Expression vector for ChrimsonR-tdTomato

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Tags

tdTomato

Expression

Mammalian

Mutation

K176R

Promoter

CAG

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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huCaV2.2 (pSAD442-1)

Plasmid

#62574

Purpose

Human CaV2.2 expression in mammalian cells

Depositor

Diane Lipscombe

Article

Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

Insert

CACNA1B (CACNA1B Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV_AncBE4max

Plasmid

#112094

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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B95(DE3) ΔA ΔfabR ΔserB

Bacterial Strain

#197655

Purpose

This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

Depositor

Richard Cooley, Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Bacterial Resistance

None

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only
pC13N-dCas9-BFP-KRAB

Plasmid

#127968

Purpose

constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-dCas9-NLS-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSBbi-GB

Plasmid

#60520

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and blasticidin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN5-ires-puro

Plasmid

#167821

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-H2-Kb SCT-T2A-GFP

Plasmid

#205454

Purpose

lentiviral plasmid for expression of mouse H2-Kb SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

H2-Kb SCT (H2-K1 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_EML4_ALK

Plasmid

#205809

Purpose

Express mEGFP-tagged fusion protein, EML4_ALK from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

EML4_ALK (EML4, ALK Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pJFRC19-13XLexAop2-IVS-myr::GFP

Plasmid

#26224

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

myr::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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