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pLenti SpBsmBI sgRNA Hygro

Plasmid

#62205

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

Depositor

Rene Maehr

Publication

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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pHAGE-TO-dCas9-3XGFP

Plasmid

#64107

Purpose

Expression of Sp dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pHAGE-TO-dCas9-3XGFP

Plasmid

#64107

Purpose

Expression of Sp dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pKSE401

Plasmid

#62202

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

Depositor

Qi-Jun Chen

Publication

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

gRNA scaffold
zCas9

Use

Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

maize codon optimized

Promoter

35S and AtU6-26p

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pKSE401

Plasmid

#62202

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

Depositor

Qi-Jun Chen

Publication

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

gRNA scaffold
zCas9

Use

Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

maize codon optimized

Promoter

35S and AtU6-26p

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pLKO5.sgRNA.EFS.tRFP657

Plasmid

#57824

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP657

Use

CRISPR and Lentiviral

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pLKO5.sgRNA.EFS.tRFP657

Plasmid

#57824

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP657

Use

CRISPR and Lentiviral

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pTARA

Plasmid

#31491

Depositor

Kathleen Matthews

Publication

Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

Insert

RNA polymerase

Expression

Bacterial

Mutation

N823D

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTARA

Plasmid

#31491

Depositor

Kathleen Matthews

Publication

Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

Insert

RNA polymerase

Expression

Bacterial

Mutation

N823D

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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AAVS1 hPGK-PuroR-pA donor

Plasmid

#22072

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PGK-Puro (PGK1 Human)

Use

Zfn donor

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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AAVS1 hPGK-PuroR-pA donor

Plasmid

#22072

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PGK-Puro (PGK1 Human)

Use

Zfn donor

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pEZYmyc-His

Plasmid

#18701

Purpose

pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

Depositor

Yu-Zhu Zhang

Publication

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Tags

Myc-His

Expression

Mammalian

Promoter

CMV

Available since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEZYmyc-His

Plasmid

#18701

Purpose

pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

Depositor

Yu-Zhu Zhang

Publication

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Tags

Myc-His

Expression

Mammalian

Promoter

CMV

Available since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV-HA-MyD88

Plasmid

#12287

Depositor

Bruce Beutler

Publication

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MyD88 (MYD88 Human)

Tags

HA

Expression

Mammalian

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pCMV-HA-MyD88

Plasmid

#12287

Depositor

Bruce Beutler

Publication

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MyD88 (MYD88 Human)

Tags

HA

Expression

Mammalian

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pCPRDEnv

Plasmid

#1732

Purpose

This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab

Depositor

Garry Nolan

Insert

Gag-Pol and Rev

Expression

Mammalian

Available since

Oct. 24, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCPRDEnv

Plasmid

#1732

Purpose

This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab

Depositor

Garry Nolan

Insert

Gag-Pol and Rev

Expression

Mammalian

Available since

Oct. 24, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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STAT3-GFP

Plasmid

#110495

Purpose

reporter of STAT3 signaling activity

Depositor

Michael Lewis

Publication

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

4 repeats of STAT3 binding site M67 at GFP promoter region

Use

Lentiviral

Expression

Mammalian

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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STAT3-GFP

Plasmid

#110495

Purpose

reporter of STAT3 signaling activity

Depositor

Michael Lewis

Publication

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

4 repeats of STAT3 binding site M67 at GFP promoter region

Use

Lentiviral

Expression

Mammalian

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-NLS-HA-Dre

Plasmid

#51272

Purpose

Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)

Depositor

Pawel Pelczar

Publication

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

Dre

Tags

NLS-HA

Expression

Mammalian

Promoter

CAG

Available since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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