168,838 results
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Plasmid#69929Purposebacterial expression of MBP-TEV n-terminal fusionsDepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsMBP and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only
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pAAV hSyn FLEx-loxp Kir2.1-2A-GFP
Plasmid#161574PurposeExpression of Kir2.1-2A-GFP in a Cre-dependent fashionDepositorAvailable SinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Parkin
Plasmid#23956PurposeMammalian expression of human Park2 fused to mCherryDepositorAvailable SinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-TFEB
Plasmid#38119PurposeMammalian expression of Transcription factor EB fused to EGFPDepositorAvailable SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-Guide
Plasmid#85401PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neoDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mRFP
Plasmid#13032PurposeMammalian expression vector for cloning your gene fused to mRFPDepositorHas ServiceCloning Grade DNAInsertMonomer Red Fluorescent Protein
ExpressionMammalianAvailable SinceNov. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
FRESCA-syntide
Plasmid#132415PurposeFRET-based sensor for CaMKII activityDepositorInsertFRESCA-syntide
ExpressionBacterial and MammalianPromoterCMVAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO mCherry (AAV Retrograde)
Viral Prep#114471-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Flp-dependent)Available SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ABEmax_P2A_GFP
Plasmid#112101PurposeA:T-to-G:C base editingDepositorInsertABEmax_P2A_GFP
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV9)
Viral Prep#26969-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsEYFPAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4
Plasmid#26103PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTags6xHis and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-GFP
Plasmid#132776PurposePrime editing in mammalian cells with co-translational GFP expressionDepositorInsertPE2-P2A-GFP
TagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-Puro
Plasmid#86708PurposeMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSGV-1D3-28Z All ITAMs intact
Plasmid#107226PurposeAnti-mouse CD19 CAR with CD3 ITAMs intactDepositorInsert1D3-28Z All ITAMs intact
UseRetroviralTagsNoneMutationNoneAvailable SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSFV3
Plasmid#92072PurposeEmpty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.DepositorTypeEmpty backboneUseEmpty expression plasmid, for run-off transcripti…Available SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-WT
Plasmid#17608PurposeMammalian expression of HA tagged ubiquitinDepositorAvailable SinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)
Plasmid#140003PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEf1a-goldDn29-dCas9-P2A-GFP
Plasmid#247157PurposeMammalian expression of a human codon optimized engineered goldDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3DepositorInsertgoldDn29-dCas9-P2A-GFP
TagsSV40 NLSExpressionMammalianMutationM6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503NPromoterEf1aAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLII12Pglu-700uDelta6
Plasmid#17866DepositorInsertFLII12Pglu-700uDelta6
TagsCitrineExpressionMammalianMutation700uDelta6, ECFP insert in mglBAvailable SinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only