168,428 results

1. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)

   Plasmid

   #191389

   Purpose

   Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hCdt1(1-100)
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD

   Plasmid

   #216248

   Purpose

   HDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSD

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   HDR template for human RANGAP1 (RANGAP1 Human)
   

   Use

   CRISPR and TALEN; Hdr template

   Tags

   miniIAA7.3xFlag.P2A.BSD

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVβ-p300.DY-myc

   Plasmid

   #30490

   Depositor

   Tso-Pang Yao

   Insert

   p300 (EP300 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   aspartic acid 1399 converted to tyrosine (Acetyla…

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pBCPB+

   Plasmid

   #18940

   Depositor

   Michele Calos

   Article

   Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

   Insert

   attP, lacZ, attB
   

   Expression

   Bacterial

   Available Since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-GRAB_SST1.0

   Plasmid

   #208665

   Purpose

   Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mOPN-pcDNA

   Plasmid

   #106447

   Purpose

   Mammalian expression of murine osteopontin

   Depositor

   Georg Weber

   Article

   Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

   Insert

   osteopontin (Spp1 Mouse)
   

   Expression

   Mammalian

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHelper_TetR/pTet-Tnpase (camR)

   Plasmid

   #213910

   Purpose

   ATc-inducible himar1C9 transposase expression - cam resistance

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   TetR/ptet ATc-inducible himar1C9 transposase
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDonor_RB-TnV2

   Plasmid

   #213907

   Purpose

   Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEM705 Gs

   Plasmid

   #109350

   Purpose

   Wildtype Gαs

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   GαS (GNAS Human)
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p663-UBC-TurboID-V5-BRSK2_IDG-K

    Plasmid

    #135272

    Purpose

    Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-BRSK2 (BRSK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. tdTomato-TOMM20-N-10

    Plasmid

    #58137

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

12. mCherry-PAK2 binding domain

    Plasmid

    #83951

    Purpose

    FLIM acceptor for mEGFP Rac1

    Depositor

    Ryohei Yasuda

    Article

    Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

    Insert

    Pak2 (PAK2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa60-113 only; S74A F84A

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-AncMEcoGII291

    Plasmid

    #205024

    Purpose

    Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 291
    

    Tags

    His6x tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-SspB-HaloTag-TIAM1-DHPH

    Plasmid

    #176127

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pES002 MBP-GSK3β_S9A-HA-His

    Plasmid

    #196184

    Purpose

    Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

    Insert

    GSK3B (GSK3B Human)
    

    Tags

    HA, His6, and MBP

    Expression

    Bacterial

    Mutation

    changed Serine 9 to Alanine

    Promoter

    Tac

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMM643

    Plasmid

    #112533

    Purpose

    pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    luxCDABE
    

    Expression

    Bacterial

    Mutation

    A17P

    Promoter

    J23107, PL(HrtO), and ProD

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pInducer10b-EGFP-KRAS-WT

    Plasmid

    #164935

    Purpose

    Expresses EGFP cDNA and KRAS wild type cDNA in mammalian cells

    Depositor

    Ji Luo

    Article

    Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

    Inserts

    Enhanced green fluorescent protein
    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    

    Use

    Lentiviral

    Promoter

    Ubc Promoter and Ubc promoter

    Available Since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYES2-HTH-HHT2

    Plasmid

    #86482

    Purpose

    yeast expression of HHT2 with His10-TEV-HA tag

    Depositor

    Akash Gunjan

    Article

    Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

    Insert

    HHT2 (HHT2 Budding Yeast)
    

    Tags

    HIS10-TEV-HA (HTH)

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Jan. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLV-hCCND1-HA

    Plasmid

    #174154

    Purpose

    Lentiviral vector expressing HA-tagged human cyclin D1

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    hemagglutinin (HA)

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-SspB-HaloTag-ITSN1-DHPH

    Plasmid

    #176128

    Purpose

    Heterodimerization with iLID, Cdc42 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    ITSN1 (ITSN1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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