168,242 results

1. Str-Ii_VSVG-SBP-EGFP

   Plasmid

   #65300

   Purpose

   synchronize trafficking of VSVG from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   VSV-G
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. spNW50

   Plasmid

   #173486

   Purpose

   Expresses spNW50 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW50
   

   Tags

   Histag

   Expression

   Bacterial

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIR-FbALFA

   Plasmid

   #184678

   Purpose

   NIR-Fb to ALFA-tag

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-FbALFA
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a-TET1-dCas9

   Plasmid

   #235593

   Purpose

   Tet1CD fused to N-terminus of dCas9

   Depositor

   Ting Zhou

   Article

   Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

   Insert

   TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)
   

   Use

   CRISPR

   Tags

   3xNLS, TET1 CD, and tagBFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mouse genome-wide lentiviral CRISPR gRNA library version 2

   Pooled Library

   #67988

   Purpose

   Mouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W)

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Species

   Homo sapiens, Mus musculus, and Synthetic

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA Myc DBC1

   Plasmid

   #35096

   Depositor

   Osamu Hiraike

   Article

   Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7.

   Insert

   deleted in breast cancer 1 (CCAR2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICH47732

   Plasmid

   #48000

   Depositor

   Sylvestre Marillonnet

   Article

   Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Dec. 3, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. Tom20-V5-FKBP-AP_pLX304

   Plasmid

   #120914

   Purpose

   Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane

   Depositor

   Alice Ting

   Article

   Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

   Insert

   Tom20-V5-FKBP-AP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV2)

   Viral Prep

   #105553-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   Syn

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

10. pRL-SV40P

    Plasmid

    #27163

    Purpose

    Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments

    Depositor

    Ron Prywes

    Article

    Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702

    Insert

    SV40 promoter
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHIV-Luc-ZsGreen

    Plasmid

    #39196

    Depositor

    Bryan Welm

    Inserts

    Firefly Luciferase Luc2P
    ZsGreen
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1-alpha and EF1-alpha

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEC3135 (pERASE)

    Plasmid

    #220306

    Purpose

    Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PP7_mCherry

    Plasmid

    #61763

    Purpose

    Expresses PP7 coat protein fused to mCherry

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    PP7 coat protein fused to mCherry
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Ubc promoter

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACYC-Skp-GroEL/ES

    Plasmid

    #83922

    Purpose

    Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Skp
    Gro EL/ES
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDD120

    Plasmid

    #119879

    Purpose

    Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pConstitutive_Che9c60 and Che9c61
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

    Plasmid

    #108871

    Purpose

    Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

    Depositor

    Alex Schier

    Article

    Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

    Inserts

    5xU6:sgRNA
    Cas9-t2A-GFP
    

    Use

    CRISPR

    Promoter

    U6 and hsp70

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mNeonGreen-mRuby2-FRET-10

    Plasmid

    #58179

    Purpose

    Localization: Cytosolic FRET, Excitation: , Emission:

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

    Type

    Empty backbone

    Tags

    mNeonGreen and mRuby2

    Expression

    Mammalian

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CAG.(mito).cpSFGFP.HaloTag

    Plasmid

    #214925

    Purpose

    Expresses mitochondrially targeted non-responsive control

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).cpSFGFP.HaloTag
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSV Sport RXR alpha

    Plasmid

    #8882

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

    Insert

    RXR alpha (RXRA Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

    Plasmid

    #222994

    Purpose

    Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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