168,956 results

1. pCVL SFFV-EF1s HA.NLS.Sce(opt)

   Plasmid

   #31479

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   SFFV/Ef1s HA NLS I-SceI
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Mutation

   "opt" indicates the coding sequence has…

   Available Since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-Rab27B

   Plasmid

   #89447

   Purpose

   Expression of GFP-tagged human Rab27B in mammalian cells

   Depositor

   Wendy Westbroek

   Article

   Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

   Insert

   human Rab27B (RAB27B Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Yarrowia lipolytica Golden Gate tool kit

   Plasmid Kit

   #1000000167

   Purpose

   Yarrowia lipolytica Golden Gate tool kit for integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica.

   Depositor

   Tristan Rossignol

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

4. pIRESpuroN3xFLAG-UFSP2 C302A

   Plasmid

   #204554

   Purpose

   Expresses 3xFLAG-tagged UFSP2 C302A in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   UFSP2 (UFSP2 Human)
   

   Expression

   Mammalian

   Mutation

   C302A

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-Tet-OsTIR1(F74G)-V5

   Plasmid

   #158664

   Purpose

   Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   Tet-OsTIR1(F74G) AAVS1
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   F74G

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFl Strep-sumo-TEV-hAgo2

   Plasmid

   #136235

   Purpose

   Expresses human Argonaute-2 in insect cells

   Depositor

   Leemor Joshua-Tor

   Article

   Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

   Insert

   human Argonaute-2 (AGO2 Human)
   

   Tags

   Strep Sumo

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET Trx His6 LIC cloning vector (2Tc-T)

   Plasmid

   #37239

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis and Thioredoxin (Trx)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2

   Plasmid

   #231372

   Purpose

   Express human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

   Depositor

   David Bartel

   Article

   Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

   Insert

   Human Argonaute2 (AGO2 Human, Synthetic)
   

   Tags

   3xFLAG-SUMO_Eu1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. p667-UBC-CDK12-V5_IDG-K

   Plasmid

   #135245

   Purpose

   Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CDK12-V5 (CDK12 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PJ23119

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTREtight2

    Plasmid

    #19407

    Depositor

    Markus Ralser

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA

    Plasmid

    #115161

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.

    Inserts

    PTRE - tet activator responsive promoter
    flex sequence
    hM3dq-mCherry (inverted)
    flex sequence
    

    Use

    AAV

    Tags

    hM3Dq-mCherry

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-EGFP MASTL WT (siRNA resistant)

    Plasmid

    #191011

    Purpose

    Expresses EGFP-tagged MASTL WT with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

    Depositor

    Johanna Ivaska

    Article

    Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Microtubule-associated serine/threonine kinase like (MASTL Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEB1-mCerulean

    Plasmid

    #103968

    Purpose

    Plasmid encoding mCerulean

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mCerulean
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    ProC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. R777-E233 Hs.RASAL1

    Plasmid

    #70517

    Purpose

    Gateway ORF clone of human RASAL1 [NM_001193521.1] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASAL1 (RASAL1 Human)
    

    Use

    Gateway entry clone

    Mutation

    R331H

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEGFP

    Plasmid

    #18696

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    monomeric EGFP
    

    Expression

    Mammalian

    Mutation

    A206K mutation

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBPZpGAL4DBDUw

    Plasmid

    #26233

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    Zip-GAL4DBD
    

    Expression

    Insect

    Available Since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT-TO-eGFP

    Plasmid

    #157733

    Purpose

    mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    eGFP
    

    Expression

    Mammalian

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMS_366

    Plasmid

    #182131

    Purpose

    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

    Depositor

    George Church

    Article

    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

    Inserts

    retron RNA
    ec.86 RT
    CspRecT
    

    Mutation

    incorporated rifampicin-resistance-conferring don…

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEM4Z-EGFP

    Plasmid

    #183475

    Purpose

    This plasmid can be used to prepare EGFP mRNA by In Vitro Transcription

    Depositor

    Chantal Pichon

    Article

    Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

    Insert

    EGFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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