Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

181,168 results

Showing: 3181 - 3200 of 181168 results

  1. pCMV-MMLVgag-3xNES-ABE8e

    Plasmid
    #181751
    Purpose
    MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.
    Depositor
    David Liu
    Publication
    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLVgag-3xNES-ABE8e
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181751-plasmid-map-sequence-id-353935

    Enlarge

  2. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Expression
    Mammalian
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    177938-plasmid-map-sequence-id-429078

    Enlarge

  3. pDF0159 pCMV - huDisCas7-11 mammalian expression

    Plasmid
    #172507
    Purpose
    Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Publication
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV - huDisCas7-11 mammalian expression
    Expression
    Mammalian
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172507-plasmid-map-sequence-id-344930

    Enlarge

  6. pAAV-hsyn-flex-Voltron-ST (AAV1)

    Viral prep
    #119036-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hsyn-flex-Voltron-ST (#119036). In addition to the viral particles, you will also receive purified pAAV-hsyn-flex-Voltron-ST plasmid DNA. Synapsin-driven, cre-dependent expression of genetically encoded fluorescent voltage indicator Voltron1 with a soma-targeting sequence. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Eric Schreiter
    Publication
    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641
    Promoter
    Syn
    Available since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only

  7. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV PHP.eB)

    Viral prep
    #104492-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  8. pENN.AAV.hSyn.TurboRFP.WPRE.RBG (AAV1)

    Viral prep
    #105552-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pENN.AAV.hSyn.TurboRFP.WPRE.RBG (#105552). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.TurboRFP.WPRE.RBG plasmid DNA. hSynapsin-driven TurboRFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    TurboRFP
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only

  12. CIDAR MoClo Parts Kit

    Plasmid kit
    #1000000059
    Purpose
    Collection of standardized parts for combinatorial design and expression tuning in E. coli.
    Depositor
    Douglas Densmore
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. pAC152-dual-dCas9VP64-sgExpression

    Plasmid
    #48238
    Purpose
    Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    HA Tag, HA-Tag, and VP64
    Expression
    Mammalian
    Mutation
    D10A;H840A
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48238-plasmid-map-sequence-id-69545

    Enlarge

  16. pUC57 APEX

    Plasmid
    #40306
    Purpose
    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pea APEX (Synthetic)
    Use
    Unspecified
    Mutation
    K14D, W41F, E112K (relative to wild-type)
    Available since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40306-plasmid-map-sequence-id-115990

    Enlarge

  18. CMV-G-GECO1.1

    Plasmid
    #32445
    Purpose
    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)
    Depositor
    Robert Campbell
    Publication
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G-GECO1.1
    Expression
    Mammalian
    Mutation
    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    32445-plasmid-map-sequence-id-150291

    Enlarge

Showing: 3181 - 3200 of 181168 results