168,238 results

1. pLPC-Myc-BirA-TRF1

   Plasmid

   #234040

   Purpose

   Expresses Myc-His-BirA(R118G)-TRF1

   Depositor

   Roderick O'Sullivan

   Article

   Garcia-Exposito et al Cell Rep. 2016 Nov 8;17(7):1858-1871. do

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Retroviral

   Tags

   Myc-6xHis-BirA(R118G)

   Expression

   Mammalian

   Available Since

   Aug. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOttc556 - pAAV EF1a iCre

   Plasmid

   #210416

   Purpose

   AAV viral vector packaging plasmid that expresses "improved Cre recombinase"

   Depositor

   Brandon Harvey, Christopher Richie

   Insert

   iCre
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167935

   Purpose

   Inducible CRISPRi expression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   KRAB-dCas9-DHFR
   

   Use

   Lentiviral

   Promoter

   tetON

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHIV-Luc-ZsGreen

   Plasmid

   #39196

   Depositor

   Bryan Welm

   Inserts

   Firefly Luciferase Luc2P
   ZsGreen
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-alpha and EF1-alpha

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW empty vector

   Plasmid

   #184708

   Purpose

   Lentiviral empty vector for doxycycline-inducible expression

   Depositor

   Alessia Ciarrocchi, Gloria Manzotti

   Article

   Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2FA CO Tol2 TPase

   Plasmid

   #133032

   Purpose

   Expression vector for production of codon optimized Tol2 transposase

   Depositor

   Charles Nathan Hancock

   Article

   Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

   Insert

   Codon optimized Tol2 Transposase
   

   Use

   In vitro expression

   Mutation

   Codon Optimized

   Available Since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. BirA pLEMO

   Plasmid

   #119818

   Purpose

   Express endogenous biotin ligase in E.coli

   Depositor

   Alexander Gabibov

   Article

   Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

   Insert

   Biotin ligase
   

   Expression

   Bacterial

   Promoter

   Rhamnose promoter

   Available Since

   April 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-2xFYVE

   Plasmid

   #140047

   Purpose

   Expresses GFP-tagged HRS 2xFYVE in mammalian cells

   Depositor

   Harald Stenmark

   Article

   Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

   Insert

   tandem HRS FYVE domain (Hgs Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   two FYVE domains (amino acids 147-223), linked by…

   Promoter

   CMV

   Available Since

   May 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFD6

   Plasmid

   #73915

   Purpose

   tissue-specific multiplex gRNA plasmid

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   :tRNA:gRNA:tRNA:gRNA:tRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   UAS-DSCP

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. M23: pHR-SFFVp-FUSN::mCherry::SspB

    Plasmid

    #122148

    Purpose

    N terminal domain of FUS (1-214) tagged with SspB and mCherry.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FUS (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-sspB

    Expression

    Mammalian

    Mutation

    Contains only amino acids 1-214

    Promoter

    SFFV

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pREDawn-AmpR-MCS

    Plasmid

    #188978

    Purpose

    For red light-induced bacterial expression of gene of interest (AmpR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTetOne-mCherry-cNLS

    Plasmid

    #138527

    Purpose

    Inducible expression of mCherry with nuclear localization signal

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    mCherry
    

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE3Gs

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-pA-Hia5

    Plasmid

    #174372

    Purpose

    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein A - hia5
    

    Tags

    6xHis and Protein A

    Expression

    Bacterial

    Available Since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRZeroT

    Plasmid

    #120276

    Purpose

    Bifunctional vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-backgound system

    Depositor

    Ken Motohashi

    Article

    Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

    Type

    Empty backbone

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. spNW100

    Plasmid

    #173488

    Purpose

    Expresses spNW100 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW100
    

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    P1049L- please see depositor comments

    Promoter

    T7

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDG459

    Plasmid

    #100901

    Purpose

    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLQ14902

    Plasmid

    #239277

    Purpose

    Expresses gG2 for pertubing endogenous human GAPDH mRNA via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PspCas13b-hGAPDH-gRNA2 (GAPDH Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ10552

    Plasmid

    #239271

    Purpose

    Expresses PYL1-G3BP1 for recruiting RNA to the stress granule via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PYL1-BFP-G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PE6a

    Plasmid

    #207851

    Purpose

    Mammalian expression of PE6a prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6a
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. mVenus C1

    Plasmid

    #27794

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Has Service

    Cloning Grade DNA

    Insert

    mVenus C1
    

    Expression

    Mammalian

    Available Since

    April 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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