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180,502 results

Showing: 3181 - 3200 of 180502 results

  1. pENN.AAV.hSyn.TurboRFP.WPRE.RBG (AAV1)

    Viral prep
    #105552-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pENN.AAV.hSyn.TurboRFP.WPRE.RBG (#105552). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.TurboRFP.WPRE.RBG plasmid DNA. hSynapsin-driven TurboRFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    TurboRFP
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only

  4. lentiGuide-Hygro-mTagBFP2

    Plasmid
    #99374
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99374-plasmid-map-sequence-id-430620

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  10. CIDAR MoClo Parts Kit

    Plasmid kit
    #1000000059
    Purpose
    Collection of standardized parts for combinatorial design and expression tuning in E. coli.
    Depositor
    Douglas Densmore
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65767-plasmid-map-sequence-id-471631

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  13. pAC152-dual-dCas9VP64-sgExpression

    Plasmid
    #48238
    Purpose
    Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    HA Tag, HA-Tag, and VP64
    Expression
    Mammalian
    Mutation
    D10A;H840A
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48238-plasmid-map-sequence-id-69545

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  15. pUC57 APEX

    Plasmid
    #40306
    Purpose
    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pea APEX (Synthetic)
    Use
    Unspecified
    Mutation
    K14D, W41F, E112K (relative to wild-type)
    Available since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40306-plasmid-map-sequence-id-115990

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  17. CMV-G-GECO1.1

    Plasmid
    #32445
    Purpose
    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)
    Depositor
    Robert Campbell
    Publication
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G-GECO1.1
    Expression
    Mammalian
    Mutation
    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    32445-plasmid-map-sequence-id-150291

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Showing: 3181 - 3200 of 180502 results