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pBABE-Stat5a1*6

Plasmid

#130668

Purpose

Retroviral vector for the expression of constitutive Stat5a (puromycin resistance)

Depositor

Gerardo Ferbeyre

Article

Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

Insert

Stat5a (Stat5a Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

changed Histidine 298 to Arginine and Serine 710 …

Promoter

LTR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-P2A-puro

Plasmid

#214358

Purpose

Expresses SpCas9 and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SpCas9 and puromycin resistance genes

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRVdGL-4Cre

Plasmid

#98039

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

mCre

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay-Rncp-iGluSnFR1

Plasmid

#107336

Purpose

Red fluorescent glutamate sensor with ncp topology. Membrane-displayed

Depositor

Robert Campbell

Article

Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

Insert

Rncp-iGluSnFR1

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-en1FnCas9

Plasmid

#201947

Purpose

Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-en1FnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

E1369R on FnCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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GPR27-Tango

Plasmid

#66349

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR27 (GPR27 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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EFNB1-bio-His

Plasmid

#51749

Purpose

Expresses full-length Ephrin-B1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

EFNB1 (EFNB1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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p5E LyzC

Plasmid

#135200

Purpose

Neutrophil specific label

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

Lyz promoter

Use

Synthetic Biology

Promoter

LyzC

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-NLS-HA-Bxb1

Plasmid

#51271

Purpose

Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-Bxb1

Expression

Mammalian

Promoter

CAG

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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phage UbiC SNAP-DDX6

Plasmid

#119948

Purpose

Lentiviral vector expressing SNAP-DDX6 fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

DDX6 (DDX6 Human)

Use

Lentiviral

Tags

SNAP

Expression

Bacterial and Mammalian

Promoter

UbiC

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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Scarlet I-FRET 10-NeonGreen

Plasmid

#139364

Purpose

FRET Standard

Depositor

Richard Day

Article

Reissaus et al Biophys J. 2020 Apr 21;118(8):1820-1829.

Insert

Scarlet 10AA Neon green

Expression

Mammalian

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pInducer20-Blast

Plasmid

#109334

Purpose

pInducer20 with added blasticidin resistance

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Type

Empty backbone

Use

Lentiviral; Inducible expression

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAM5406

Plasmid

#132661

Purpose

RSF1010-based broad host-range plasmid with additional RK2 bom site and pUC origin

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyCatcher

Plasmid

#72326

Purpose

Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

AviTag-SpyCatcher

Tags

N-term His6 tag and TEV cleavage site

Expression

Bacterial

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_WT

Plasmid

#81892

Purpose

Gateway Donor vector containing ERBB2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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8xNFAT-ZsG-muhCD4

Plasmid

#162745

Purpose

NFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene

Depositor

Maki Nakayama

Article

Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Human CD4 gene with glutamine to tyrosine at posi…

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+/mit-2mutAEQ

Plasmid

#45539

Depositor

Javier Alvarez-Martin

Article

de la Fuente et al Biochem J. 2012 Aug 1;445(3):371-6. doi:

Insert

mit-2mutAEQ

Tags

Mitochondrially targeted

Expression

Mammalian

Mutation

Asp119Ala and Asn28Leu

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX4T2 RANK-L

Plasmid

#108571

Purpose

Encodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tag

Depositor

Jean-Claude Scimeca

Article

Beranger et al J Bone Miner Res. 2007 Jul;22(7):975-83.

Insert

RANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)

Tags

GST

Expression

Mammalian

Mutation

aa158-aa316

Promoter

Ptac (trp/lac)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pStreptagIIx2 Alfatag 3C InsR EABR

Plasmid

#234996

Purpose

For production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surface

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

InsR-EABR (INSR Human)

Tags

2xStreptag-II, Alfatag, HRV 3C site

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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