Addgene

We narrowed to 5,442 results for: arl

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pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84241

Purpose

Expresses ABA-inducible KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Mutation

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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GST-mDia1(FH1FH2DAD)-His

Plasmid

#85822

Purpose

contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.

Depositor

Guillaume Romet-Lemonne, Antoine Jégou

Article

Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc

Insert

Protein diaphanous homolog 1 (Diaph1 Mouse)

Use

Tags

8*His and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pG2J

Plasmid

#102882

Purpose

Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Inserts

OsTIR1
mGFP-AID

Use

Tags

AID(71-114)

Expression

Yeast

Mutation

Promoter

ADH1 and TEF1

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-CFL-SN

Plasmid

#181740

Purpose

AAV vector for expressing CFL-SN

Depositor

Yasunori Hayashi

Article

Goto et al Science. 2021 Nov 12;374(6569):857-863.

Insert

CFL-SN

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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Stargazin-dCherry-FRB

Plasmid

#172444

Purpose

Expression of plasma membrane protein Stargazin tagged with dark (non-fluorescent) mCherry-FRB

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cacng2 (Cacng2 Mouse)

Use

Tags

dCherry-HA-FRB

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLB038 - PHS1∆P

Plasmid

#136285

Purpose

entry vector for GreenGate cloning method, C module, open reading frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) (PHS1DP)

Depositor

Alexis Maizel

Article

Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

Insert

PHS1∆P

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-EEA1

Plasmid

#176491

Purpose

For lentiviral transduction of 3FLAG-EEA1

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

Plasmid

#216164

Purpose

Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function

Depositor

Pietro Fratta

Article

Wilkins et al bioRxiv. 2023 Nov 15:2023.11.15.565967.

Insert

Gaussia princeps luciferase (with double mutation to improve stability)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS_L_MARV

Plasmid

#135473

Purpose

Mammalian expression vector encoding Marburg virus L gene under control of the AG promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

L gene

Use

Tags

Expression

Mammalian

Mutation

silent mutation (C to T) at position 7861; insert…

Promoter

AG

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84239

Purpose

Expresses ABA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Mutation

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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SOX17-3'gRNA

Plasmid

#210465

Purpose

gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

SOX17 (SOX17 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-eFFly-eGFP

Plasmid

#104834

Purpose

eFFly luciferase for invivo Imaging, eGFP for flourescent detection

Depositor

Irmela Jeremias

Article

Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

Inserts

eFFly
eGFP

Use

Lentiviral and Luciferase; Egfp

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-synaptoPAC-minWPRE

Plasmid

#153100

Purpose

Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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DYRK1A/pTRE3G-FL-hXIST

Plasmid

#149608

Purpose

Express doxycycline-inducible full length of human XIST cDNA

Depositor

Jeanne Lawrence

Article

Jiang et al Nature. 2013 Aug 15;500(7462):296-300. d

Insert

X-inactive specific transcript (XIST Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pTRE3G

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ZFP36

Plasmid

#178438

Purpose

doxycycline-inducible expression of Human ZFP36 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

ZFP36 (ZFP36 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-E2

Plasmid

#194590

Purpose

Bacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

E2 (S )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP Abi1

Plasmid

#74905

Purpose

Expresses human Abi1 fused to GFP

Depositor

Giorgio Scita

Article

Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

Insert

Abi1 (ABL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-dLight1.3b

Plasmid

#135762

Purpose

For expression of the genetically encoded dopamine sensor dLight1.3b

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-EFIa-Id1-PGK-puro

Plasmid

#107735

Purpose

Expresses Id1 in mammalian cells

Depositor

Alexandre Colas

Article

Cunningham et al Genes Dev. 2017 Aug 9. pii: gad.300400.1

Insert

Id1 (T Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-human APPL1

Plasmid

#22198

Depositor

Pietro De Camilli

Article

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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