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Plasmid#159749PurposeUbiquitin promoter-controlled expression of 3ĆFLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD
Plasmid#225703PurposeLentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mTurquoiseExpressionMammalianMutationK36MPromoterpEF1AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD
Plasmid#225702PurposeLentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mTurquoiseExpressionMammalianMutationK27MPromoterpEF1AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.CBX8ĪChromo
Plasmid#224708PurposeExpresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tagDepositorInsertCBX8 chromodomain truncation (CBX8 Human)
UseTagsHMBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.mEGFP-CBX8
Plasmid#224710PurposeExpresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.DepositorInsertCBX8 (CBX8 Human)
UseTagsmEGFPExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFB1.HMBP.PrS.CBX8-KR21A
Plasmid#224709PurposeExpresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertCBX8 KR21A mt (CBX8 Human)
UseTagsHMBPExpressionInsectMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.CBX8
Plasmid#224707PurposeExpresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertCBX8 (CBX8 Human)
UseTagsHMBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)
Plasmid#194234PurposeLentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-Spike ECD-PDGFR TMD-T2A-mCherry (S Synthetic)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)
Plasmid#223166Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOEDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER
Plasmid#222189PurposeBacterial expression of mouse PTER proteinDepositorInsertPTER (Pter Mouse)
UseTags6xHis and StrepExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
LMPd Ametrine Got1 shRNA#1
Plasmid#220592PurposeRetroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffoldDepositorInsertGot1 shRNA (Got1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcdna 3.1 DSG-3 Tailless Control
Plasmid#182718Purposeexpresses a truncated force-insensitive DSG-3 TSmodDepositorInsertdesmoglein-3 (Dsg3 Mouse)
UseTagsExpressionMammalianMutationTSmod inserted at G726, downstream C-terminal taiā¦PromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
LMPd Ametrine Got2 shRNA#3
Plasmid#220593PurposeRetroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffoldDepositorInsertGot2 shRNA (Got2 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT222 lenti pEF-rTetR(SE-G72P)-3XFLAG-FOXO3 FOXO-TAD-T2A-mCherry-BSD-WPRE
Plasmid#188760PurposeLentiviral backbone containing FOXO3 FOXO-TAD as a fusion with rTetR(SE-G72P)-3XFLAGDepositorArticleInsertFOXO3 FOXO-TAD (FOXO3 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT236 lenti pEF-rTetR(SE-G72P)-3XFLAG-CXXC1 PHD-T2A-mCherry-BSD-WPRE
Plasmid#188764PurposeLentiviral backbone containing CXXC1 PHD as a fusion with rTetR(SE-G72P)-3XFLAGDepositorArticleInsertCXXC1 PHD (CXXC1 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMV5 lenti pEF-rTetR(SE-G72P)-3XFLAG-APBB1(WW)-T2A-mCherry-BSD-WPRE
Plasmid#188751PurposeLentiviral backbone containing APBB1 WW domain as a fusion with rTetR(SE-G72P)-3XFLAGDepositorArticleInsertAPBB1 WW (APBB1 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMV6 lenti pEF-rTetR(SE-G72P)-3XFLAG-CRTC2(TORC-C)-T2A-mCherry-BSD-WPRE
Plasmid#188752PurposeLentiviral backbone containing CRTC2 TORC_C domain as a fusion with rTetR(SE-G72P)-3XFLAGDepositorArticleInsertCRTC2 TORC_C (CRTC2 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMV7 lenti pEF-rTetR(SE-G72P)-3XFLAG-CACO1(Zn-C2H2_12)-T2A-mCherry-BSD-WPRE
Plasmid#188753PurposeLentiviral backbone containing CACO1 Zn-C2H2_12 domain as a fusion with rTetR(SE-G72P)-3XFLAGDepositorArticleInsertCACO1 Zn-C2H2_12 (CALCOCO1 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
LacI-pLacI-pI pm
Plasmid#218098PurposePhagemid that expresses LacI, and pI under the Lac promoterDepositorInsertpI
UseTagsExpressionBacterialMutationPromoterLac promoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
LacI-pLacI-pII pm
Plasmid#218099PurposePhagemid that expresses LacI, and pII under the Lac promoterDepositorInsertpII
UseTagsExpressionBacterialMutationPromoterLac promoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only