We narrowed to 3,913 results for: mak

1. pENTR_AtU6p_BsaI_ccdb_gRNA

   Plasmid

   #225986

   Purpose

   To make Gateway entry clone of CRISPR guide RNA under AtU6p

   Depositor

   Imran Siddiqi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCENTR_DMC1p_amiRNA

   Plasmid

   #225985

   Purpose

   To drive any artificial microRNA under the regulatory control of AtDMC1 promoter and it will make a gateway entry clone

   Depositor

   Imran Siddiqi

   Type

   Empty backbone

   Use

   RNAi

   Expression

   Plant

   Available Since

   Oct. 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. mProx1

   Plasmid

   #87129

   Purpose

   To make in situ hybridization probe for mouse Prox1.

   Depositor

   Francesca Spagnoli

   Insert

   Prox1 (Prox1 Mouse)
   

   Expression

   Mammalian

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. RPL3-GFP (96)

   Plasmid

   #24040

   Depositor

   Michael Rout

   Insert

   RPL3 (RPL3 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

5. Hsp68-CreER(TM) (DM#264)

   Plasmid

   #15028

   Depositor

   Douglas Melton

   Insert

   Cre-ER(TM)
   

   Use

   Cre/Lox; For making transgenic constructs

   Tags

   Hsp68 promoter and polyA

   Expression

   Mammalian

   Available Since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. 2PSW

   Plasmid

   #51342

   Depositor

   Cheryl Arrowsmith

   Insert

   2PSW
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Truncation: See Comments

   Promoter

   T7

   Available Since

   Feb. 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pBS AID-3xF_PP

   Plasmid

   #235502

   Purpose

   Vector to make C-Term AID fusions

   Depositor

   Brian Hendrich

   Insert

   Auxin Inducible Degron
   

   Use

   Mouse Targeting

   Tags

   AID, 3xFLAG

   Mutation

   None

   Promoter

   PGK

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZP06B

   Plasmid

   #232321

   Purpose

   Make sacB a counterselection marker to replace galK

   Depositor

   Chenggang Wu

   Insert

   PrpsJ-sacB
   

   Expression

   Bacterial

   Available Since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK33-GW-TurboID-V5

   Plasmid

   #196281

   Purpose

   Gateway destination vector to express C-terminal TurboID fusion under metallothionein promoter, for making stable cell lines

   Depositor

   Norbert Perrimon

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK33-V5-TurboID-GW

    Plasmid

    #196282

    Purpose

    Gateway destination vector to express N-terminal TurboID fusion under metallothionein promoter, for making stable cell lines

    Depositor

    Norbert Perrimon

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mOrange in pBSDONR P4r-P2

    Plasmid

    #121529

    Purpose

    For making C-terminal mOrange fusions using multisite Gateway cloning

    Depositor

    Roger Innes

    Insert

    mOrange
    

    Expression

    Bacterial

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWALIUM10-GW-TurboID-V5

    Plasmid

    #196283

    Purpose

    Gateway destination vector to express C-terminal TurboID fusion under UAS promoter, for making transgenic flies

    Depositor

    Norbert Perrimon

    Type

    Empty backbone

    Use

    Gateway destination vector

    Expression

    Insect

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWALIUM10-V5-TurboID-GW

    Plasmid

    #196285

    Purpose

    Gateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic flies

    Depositor

    Norbert Perrimon

    Type

    Empty backbone

    Use

    Gateway destination vector

    Expression

    Insect

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBCG10

    Plasmid

    #232323

    Purpose

    Make mazF a counterselection marker to replace sacB in pZP06C

    Depositor

    Chenggang Wu

    Insert

    mazF-tetR
    

    Expression

    Bacterial

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. sCFP in pBSDONR P4r-P2

    Plasmid

    #121530

    Purpose

    For making C-terminal sCFP fusions using multisite Gateway cloning

    Depositor

    Roger Innes

    Insert

    sCFP
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRetroQ-Cre-ERT2

    Plasmid

    #59701

    Purpose

    to make Cre-ERT2 expressing stable cell line by retrovirus

    Depositor

    Richard Youle

    Insert

    Cre-ERT2
    

    Use

    Retroviral

    Tags

    Estrogen receptor (ERT)

    Expression

    Mammalian

    Promoter

    cmvIE

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. Sperm whale Mb 64Y68F in pVP80K

    Plasmid

    #86956

    Purpose

    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).

    Depositor

    John Olson

    Insert

    Mb 64Y68F
    

    Expression

    Bacterial

    Mutation

    64Y68F

    Promoter

    T5

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. mouse ptc probe M2-3

    Plasmid

    #58701

    Purpose

    makes mouse ptc probe for in situ hybridization

    Depositor

    Matthew Scott

    Insert

    ptc (Ptch1 Mouse)
    

    Use

    In situ probe

    Expression

    Bacterial

    Mutation

    only EcoRI fragment (5' end)

    Promoter

    T7

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pb-CAGGS-dCas9-blasto

    Plasmid

    #218555

    Purpose

    Encodes CAG promoter-driven dCas9 with a C-terminal bipartite SV40 NLS, along with blasticidin resistance

    Depositor

    Alejandro Chavez

    Insert

    dCas9
    

    Use

    CRISPR; Piggybac transposition

    Tags

    2xSV40NLS

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG1guide_RUBY_EggCas9

    Plasmid

    #225983

    Purpose

    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants

    Depositor

    Imran Siddiqi

    Insert

    Guide RNA against AtAGAMOUS1
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    35s promoter to Drive RUBY and DD45p to drive Cas9

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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