-
Plasmid#108235PurposeBroad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.DepositorInsertPc promoter - mTurquoise2
UseTagsExpressionBacterialMutationcodon optimized for experession in P. fluorescensPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-miR-34 WT
Plasmid#78258PurposepsiCHECK2 dual luciferase reporter harboring a fully complementary miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertfully complimentary miR-34 target site
UseLuciferase; Microrna activityTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-RiboL1-jGCaMP8s
Plasmid#192611PurposeAAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8s.DepositorInsertRiboL1-jGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ-5S-F30-Control
Plasmid#127582PurposeExpresses 5S-F30-Control rRNA from a mU6 promoterDepositorInsert5S rRNA with F30 folding scaffold
UseTagsExpressionMammalianMutationPromotermodified U6Available sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Twitch-4 pcDNA3
Plasmid#49533PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-4
UseTagsECFP and cpCit174ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
VAMP3-mCherry
Plasmid#92423PurposeExpression of VAMP3 C-terminally conjugated to mCherry.DepositorInsertVAMP3 (Vamp3 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 EDD
Plasmid#37190DepositorInsertEDD (UBR5 Human)
UseTags6x His Tag and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-JEDI-1P-WPRE
Plasmid#202616PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for astrocytes specific expression using the promoter GFAPDepositorInsertJEDI-1P
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6011nes
Plasmid#108652PurposeAn ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-ERK-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dPlcR(K30/32L)
Plasmid#181833PurposePhospholipid binding-deficient Lyn-dPlcR negative-control plasmid.DepositorInsertLyn-dPlcR (K30L,K32L)
UseTagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationLysines 30 and 32 in PH domain mutated to leucine.PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSW002-PpsbA-DsRed-Express2
Plasmid#111257PurposeBroad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with DsRed-Express2DepositorInsertDsRed-Express2
UseTagsExpressionBacterialMutationPromoterPpsbAAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCA15-Ubc-NLS- scFV-tdTomato
Plasmid#199446PurposeExpression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cellsDepositorInsertscFV-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
cmv-sfGFP-GB1-scAB-ubc-nls-pcp-10xSunTag(SRv1.1-2P)
Plasmid#185801Purposeone plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.1DepositorInsertcmv-sfGFP-GB1-scAB--ubc-nls-pcp-10xSunTag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry2-mito
Plasmid#164164PurposeExpresses mCherry2-fused with a mitochondria-targeting signal of the ActA gene of Listeria monocytogenes under the control of CAG promoterDepositorInsertmCherry2-mtActA
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Rap1
Plasmid#134928PurposeFluorescently tagged GTPase.DepositorInsertRap1B, Cerulean
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mClover3-18aa-actin
Plasmid#74259PurposeMammalian expression of N-terminal fusion of mClover3 to actinDepositorInsertActin
UseTagsmClover3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHsp83-NLS-HA-2xMCP-2xTagRFP-T
Plasmid#71242PurposeUsed to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.DepositorInsertNLS-2xMCP-2xTagRFP-T
UseP element-mediated germline transformationTagsTagRFP-TExpressionInsectMutationPromoterHsp83Available sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
GW1-mito-mCherryEA
Plasmid#207868PurposeMitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensorDepositorInsertmito-mCherryEA
UseTags4xCox8 mitochondrial targetingExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)
Plasmid#128608PurposeExpresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsert36xMashTag
UseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only