Addgene

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FEMP

Plasmid

#191973

Purpose

FRET-based indicator of ER-mitochondria proximity (FEMP)

Depositor

Luca Scorrano

Article

Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

AkapYFP-Tav2a-CFP-sac1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAS1yl

Plasmid

#73226

Purpose

Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells

Depositor

Sheng Yang

Article

Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

Inserts

Cas9
sgRNA

Use

CRISPR; In y. lipolytica

Tags

Expression

Bacterial

Mutation

Promoter

unknown

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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TC1405

Plasmid

#164877

Purpose

BFP-preSTOP-GFP reporter

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

BFP-preSTOP-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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p6642 MSCV-P C-FlagHA 6bE7-Kozak

Plasmid

#35020

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV6b E7 (E7 HPV 6b)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

Plasmid

#162335

Purpose

Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Electra1-P2A-EGFP

Plasmid

#184935

Purpose

Expresses blue fluorescent protein Electra1 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1-P2A-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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p6592 MSCV-IP N-HAonly WWP2

Plasmid

#34905

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

WWP2 (WWP2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5

Plasmid

#60532

Purpose

Donor vector for human Pak5

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
TC1078-30

Plasmid

#164878

Purpose

30bp(mismatch17) cas13d gRNA for 1405 editing

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

gRNA for TC1405

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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p6644 MSCV-P C-Flag HA 45E7-Kozak

Plasmid

#35135

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV45 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-EBFP2-P2A-EGFP

Plasmid

#184938

Purpose

Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

EBFP2-P2A-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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p6656 MSCV-P C-Flag HA 25E7-Kozak

Plasmid

#35149

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV25 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6652 MSCV-P C-Flag HA 8E7-Kozak

Plasmid

#35145

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV8 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
p6653 MSCV-P C-Flag HA 92E7-Kozak

Plasmid

#35146

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV92 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6654 MSCV-P C-Flag HA 98E7-Kozak

Plasmid

#35148

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV98 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6651 MSCV-P C-Flag HA 76E7-Kozak

Plasmid

#35144

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV76 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6649 MSCV-P C-FlagHA 38 E7-Kozak

Plasmid

#35143

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV38 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6645 MSCV-P C-Flag HA 57E7-Kozak

Plasmid

#35136

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV57 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6641 MSCV-P C-FlagHA 18E7-Kozak

Plasmid

#35019

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV18 E7 (E7 HPV 18)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

March 14, 2012

Availability

Academic Institutions and Nonprofits only
plRL118

Plasmid

#163632

Purpose

Renilla luciferase reporter plasmid; Giles integrating.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Mycobacterial Optimized Promoter (MOP)

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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