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Plasmid#162308PurposeLevel 0 C-terminal tag part for MoClo assembly, TTCG-GCTTDepositorInsertC-terminal tag, HiBiT-stop, frame2 (TTC-G)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0281
Plasmid#162302PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, [S-tag]-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas12c-sgRNA_BbsI_CAM
Plasmid#183221PurposeCas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertCas12c single guide RNA containing BbsI sites in spacer sequence
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-RFP
Plasmid#35281DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092709
Plasmid#153859PurposeBacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTRDepositorInsertfragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationNearly native encoding;handful SNPs as watermarks…PromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBbB8c-GFP
Plasmid#35362DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBADAvailable sinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJBL7026
Plasmid#128811PurposeFluoride riboswitch-regulated 3-way junction dimeric broccoliDepositorInsertFluoride riboswitch-regulated 3-way junction dimeric broccoli
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHPdesteGFP
Plasmid#24566DepositorInsert
UseReporter constructTagseGFPExpressionMutationPromoterAvailable sinceMay 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJL005
Plasmid#92327PurposeExpress FtsZ tagged with mEos3.2 in E coliDepositorInsertFtsZ
UseTagsmEos3.2ExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTrc_AraC-I113-LOV
Plasmid#206804PurposeExpresses AraC-I113-LOV from a Trc promoter. For use in E. coliDepositorInsertAraC-AsLOV2 fusion
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrc_AraC-S170-LOV
Plasmid#206805PurposeExpresses AraC-S170-LOV from a Trc promoter. For use in E. coliDepositorInsertAraC-AsLOV2 fusion
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB401NL3F10H
Plasmid#141285PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL5
Plasmid#72989PurposeRBS_M2DepositorInsertM2 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL11
Plasmid#72995PurposeRBS_L4DepositorInsertL4 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TDGS50
Plasmid#183189PurposeTEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoterDepositorInsertsDivIVA-GFP(tevR M0050)
TEV-S219V
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah2
Plasmid#122569PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
RAD Module G8-C1
Plasmid#38208DepositorInsertsSET Integrase
Bxb1 Excisonase
RESET Integrase
UseSynthetic Biology; Biobrick cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbA1c-RFP
Plasmid#35335DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterTrcAvailable sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL0_4 [pIJ101 ORI]
Plasmid#198921PurposeLevel 0 partDepositorInsertpIJ101 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPTK001-2-pAOX1
Plasmid#84960PurposeType 2, pAOX1, Alcohol oxidase 1 promoterDepositorInsertAlcohol oxidase 1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only