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Plasmid#78260PurposepsiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertfully complimentary let-7 target site
UseLuciferase; Microrna activityTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
CALID
Plasmid#169708PurposeCre-activated light-inducible Dre recombinaseDepositorInserta double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1651-sgTelomere(F+E)
Plasmid#51024PurposeLentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeresDepositorInsertOptimized sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138133PurposeExpresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertEGFP-anti-rabbit-IgG(Fc)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-calpainsensor
Plasmid#36182Purposedetection of activity of ubiquitous calpainsDepositorInsertalpha-Fodrin cleavage site (Sptan1 Mouse)
UseTagseCFP and eYFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag
Plasmid#132397Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1058APromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin
Plasmid#134860PurposeExpresses EGFP-tagged recycling endosome markerDepositorInsertRAB11B (RAB11B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 F-tractin-EGFP
Plasmid#58473PurposeExpresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoterDepositorInsertF-tractin (Itpka Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-HRas
Plasmid#18662DepositorInsertH-Ras (HRAS Human)
UseTagsmonomeric EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2
Plasmid#203229PurposeCre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.DepositorInsertCre-dependent, membrane-localized Optopatch
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mGFP-Actin
Plasmid#21948DepositorInsertbetaActin (ACTB Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:myrGCaMP6s-P2A-H2AmCherry
Plasmid#170209PurposeDrives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleusDepositorInsertslc1a3b (slc1a3b Zebrafish)
UseZebrafishTagsmyrGCaMP6s-P2A-H2AmCherryExpressionMutationPromoterslc1a3bAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_eCB2.0
Plasmid#164604PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in neuronsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVTagsExpressionMutationS383TPromoterhSynAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
SynTagMA_pre
Plasmid#119738PurposeSynaptic Tag for Mapping ActivityDepositorInsertSynTagMA_pre
UseAAVTagsExpressionMammalianMutationPromoterhsyn1Available sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEV53D
Plasmid#44168DepositorInsertEquine infectious anemia virus (EIAV) gag-pol,rev, and S2
UseLentiviralTagsExpressionMammalianMutationA131T mutation in gag; Deletion of viral LTR sequ…PromoterCMVAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP
Plasmid#164038PurposeExpression of dCas9-14xGFP11 in mammalian cellsDepositorInsertdCas9-14xGFP11
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and H840A in Cas9PromoterdSV40Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
SynTagMA_post
Plasmid#119736PurposeSynaptic Tag for Mapping ActivityDepositorInsertSynTagMA
UseAAVTagsFLAGExpressionMammalianMutationPromoterhSyn-1Available sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-ER-5
Plasmid#55836PurposeLocalization: Endoplasmic Reticulum, Excitation: 558, Emission: 583DepositorInsertER (CALR Human)
UseTagsDsRed2ExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
hATMS1981A
Plasmid#32300DepositorInsertATM (ATM Human)
UseTagsFlag and HisExpressionMammalianMutationS1981APromoterAvailable sinceNov. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2
Plasmid#203228PurposeCo-expressing membrane-localized Voltron2 and membrane-localized CheRiff.DepositorInsertMembrane-localized optopatch for neuronal dendrites
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only