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Plasmid#183674PurposePtdIns(3,4)P2 biosensor for purification from bacteriaDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseTagsHis6-EGFPExpressionBacterialMutationAmino acids 169-329PromoterT7Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
SopB(C460S)-EGFP
Plasmid#183656PurposeC-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expressionDepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium)
UseTagsEGFPExpressionMammalianMutationC460SPromoterCMVAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SopB(C460S)
Plasmid#183658PurposeN-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expressionDepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium)
UseTagsEGFPExpressionMammalianMutationC460SPromoterCMVAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
BiDi-[mp-mEGFP]mp-mCherry2
Plasmid#182870PurposeBi-directional expression vector of monomers with plasma membrane localization signalDepositorInsertmyr-palm-mEGFP, myr-palm-mCherry2
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-mEGFP(4x)
Plasmid#182873PurposeHomo-tetramer expression vector with plasma membrane localization signalDepositorInsertmyr-palm-mEGFP(4x)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-eGFP::GW,attB}
Plasmid#182167Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{ie1-eGFP::GW,attB}
Plasmid#182172Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EGFP.T2A.NCLX.3'UTR
Plasmid#181872PurposeExpresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)DepositorInsertsUseAAV and AdenoviralTagsHA, T2A, and mycExpressionMammalianMutationincludes a large portion of the Slc8b1 3'UTR…Promotersynthetic hybrid CAG promoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3-FLuc-5BoxB_O
Plasmid#147079PurposeMammalian Expression of 5BoxBDepositorInsert5BoxB
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-EGFP
Plasmid#174477PurposeExpress EGFP with actin-targeting sequence in mammalian cellsDepositorInsertEGFP with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-POLBHR-eGFP
Plasmid#176064PurposeHomology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1DepositorInsertEGFP-PolB (POLB Human)
UseTagsEGFPExpressionMammalianMutationpUC with endogenous SapI mutated out, PAM mutatio…PromoterN/AAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
CTAG_eGFP-GHtag-HDEL
Plasmid#136096PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tagDepositorInsertCTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-Cavin4b-EGFP
Plasmid#176019PurposeLeishmania cell free expression of zebrafish Cavin4b-EGFP. Parton lab clone GRJDepositorInsertCavin4b (cavin4b Zebrafish)
UseLeishmania cell free expressionTagsExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-EGFP-Bin1a
Plasmid#176020PurposeLeishmania cell free expression of zebrafish EGFP-Bin1a. Parton lab clone GRQDepositorInsertBin1a
UseLeishmania cell free expressionTagsEGFPExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-EGFP-Bin1b
Plasmid#176021PurposeLeishmania cell free expression of zebrafish EGFP-Bin1b. Parton lab clone GRSDepositorInsertBin1b (bin1b Zebrafish)
UseLeishmania cell free expressionTagsEGFPExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-Cavin4a-EGFP
Plasmid#176018PurposeLeishmania cell free expression of zebrafish Cavin4a-EGFP. Parton lab clone GRKDepositorInsertCavin4a (cavin4a Zebrafish)
UseLeishmania cell free expressionTagsExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP*
Plasmid#175238PurposeBacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the KmDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP
Plasmid#175236PurposeBacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycinDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-EGFP-ratCENP-C
Plasmid#174445Purposein vitro transcription template for rat CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-EGFP-pumilioCENP-C
Plasmid#174446Purposein vitro transcription template for R. pumilio CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only