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TFORF3086

Plasmid

#144562

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

STAT1 (STAT1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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PLVX_SPTA1_IGF2BP1_IRES_PURO

Plasmid

#100016

Purpose

To over-express IGF2BP1 in Adult Erythroblasts using tissue specific promoter with IRES for puromycin selection.

Depositor

Jeffery Miller

Article

de Vasconcellos et al Proc Natl Acad Sci U S A. 2017 Jul 11;11

Insert

Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SPTA1 erythroid specific promoter

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FGFR2c-V5/HIS

Plasmid

#201107

Purpose

expression of human FGFR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMS-dsRed2-WT LMNA-GFP minigene

Plasmid

#128230

Purpose

Lamin A splicing reporter

Depositor

Tom Misteli

Article

Shilo et al Nucleic Acids Res. 2019 Apr 22. pii: 547

Insert

lamin A (LMNA Human)

Use

Tags

GFP and SV40-driven dsRed2 to identify transfecte…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2

Plasmid

#59904

Purpose

Expresses 6xHis-Ago2 (N-termi tag) in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCW57.1 blast OFF 3xflag IREB2

Plasmid

#169889

Purpose

Express 3x flag IREB2, suppressible by doxycycline addition

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

IREB2 (IREB2 Human)

Use

Lentiviral; Doxycycline mediated suppression

Tags

3x Flag

Expression

Mammalian

Mutation

Silent mutations in sgIREB2_1 and sgIREB2_2 targe…

Promoter

TRE

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EED

Plasmid

#125163

Purpose

Expresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein EED (EED Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Lrp6

Plasmid

#123595

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

low density lipoprotein receptor-related protein 6 (Lrp6 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

Plasmid

#115788

Purpose

Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

LRP5 (LRP5 Human)

Use

Tags

3xflag

Expression

Mammalian

Mutation

contains silent mutation that creates an internal…

Promoter

CMV

Available since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-METTL14-WT

Plasmid

#141112

Purpose

Overexpression of WT METTL14 in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

HMETTL14 (METTL14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS Donor mClover-LMNA

Plasmid

#98970

Purpose

Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pFl Strep-sumo-TEV-hAgo2 D597N

Plasmid

#136236

Purpose

Expressing catalytically inactive human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Article

Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

Insert

human Argonaute-2 (AGO2 Human)

Use

Tags

Sterp Sumo

Expression

Insect

Mutation

D597N

Promoter

polyhedrin

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro

Plasmid

#132773

Purpose

Expression of human LMNA.

Depositor

Jan Lammerding

Article

Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BMPR1A_pLX307

Plasmid

#98320

Purpose

Lentiviral expression of BMPR1A

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

BMPR1A (BMPR1A Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET32bplus_trxAChEwt

Plasmid

#83916

Purpose

Encodes an E. coli codon optimized version of wild type human acetylcholinesterase

Depositor

Sarel Fleishman

Article

Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:

Insert

Short form of hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)

Use

Tags

His, S-tag, and TRX

Expression

Bacterial

Mutation

codon optimised for E.coli expression

Promoter

T7 promoter

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT

Plasmid

#204694

Purpose

This plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-NUT (BRD4, NUTM1 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

direct fusion of BRD4 and NUT

Promoter

CMV

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Golgi-RasAR

Plasmid

#205570

Purpose

Golgi membrane-targeted RasAR.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

Golgi-RasAR (RAF1, GOLGB1 Human, Synthetic)

Use

Tags

Golgin B1 (Giantin) residues 3131-3259

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pT3-EFa1-HA-Jag1

Plasmid

#46051

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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BRD4 gRNA (BRDN0001147320)

Plasmid

#77344

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD4 (BRD4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BRD4 gRNA (BRDN0001147232)

Plasmid

#77345

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD4 (BRD4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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