Addgene

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pDONR223_FYN_WT

Plasmid

#82211

Purpose

Gateway Donor vector containing FYN , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FYN (FYN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Bassoon scFv [L124/59]

Plasmid

#190508

Purpose

Mammalian Expression of Bassoon scFV. Derived from hybridoma L124/59.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Bassoon (Rattus norvegicus) recombinant scFV (Bsn Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZc-HsACTB*-thymosinB-8His

Plasmid

#111146

Purpose

Expresses human beta-actin fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

ACTB (ACTB Human)

Use

Pichia pastoris integration

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

Promoter

AOX1

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

Plasmid

#181853

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_DNAJB1_PRKACA

Plasmid

#205805

Purpose

Express mEGFP-tagged fusion protein, DNAJB1_PRKACA from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

DNAJB1_PRKACA (DNAJB1, PRKACA Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti.pFOXA2.5'.GFP

Plasmid

#59407

Purpose

expresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

Depositor

Kai-Christian Sonntag

Article

Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

Insert

approx. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

FOXA2

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-pEGFP(isoform 2)

Plasmid

#99562

Purpose

mammalian expression of TMEM230-GFP (isoform 2)

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-SKM

Plasmid

#136551

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2-T2A-Klf4-E2A-cMyc (Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-ITGB1BP3

Plasmid

#23393

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ITGB1BP3 (NMRK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
TMEM230-WT-pEGFP(isoform 1)

Plasmid

#99561

Purpose

mammalian expression of TMEM230-GFP (isoform 1)

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Contactin/F3 [K73/20R]

Plasmid

#177447

Purpose

Mammalian Expression Plasmid of anti-Contactin/F3 (Rat). Derived from hybridoma K73/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Contactin/F3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Cntn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1MKK7B2

Plasmid

#19731

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

Jnk1a1MKK7b2 (Map2k7, MAPK8 Mouse, Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
Zebrafish ArcLight Q175

Plasmid

#53616

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Zebrafish ArcLight-Q175 (tpte Aequorea victoria, Synthetic, Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Dr-VSD contains R153Q mutation and an amino acid …

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-MADCAM1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156737

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

MADCAM1 (MADCAM1 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CNTN1-Fc(DAPA)-AviTag-6xHis

Plasmid

#157012

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CNTN1 (CNTN1 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-EGR3-P2A-mCherry

Plasmid

#203861

Purpose

Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

EGR3 (EGR3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Bassoon [L124/59R]

Plasmid

#177470

Purpose

Mammalian Expression Plasmid of anti-Bassoon (Rat). Derived from hybridoma L124/59.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Bassoon (Rattus norvegicus) recombinant mouse monoclonal antibody (Bsn Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-TTK

Plasmid

#23857

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TTK (TTK Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
TMEM230-WT-IRES(isoform 2)

Plasmid

#99564

Purpose

mammalian expression of TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pPICZc-Ub-R-HsACTB*-thymosinB-8His

Plasmid

#111149

Purpose

Expresses arginylated human beta-actin fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

ACTB (ACTB Human)

Use

Pichia pastoris integration plasmid

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

N-terminal arginylation

Promoter

AOX1

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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