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Plasmid#133072PurposeEntry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.DepositorTypeEmpty backboneUseEntry vectorTags3C-Flag-TwinStrep and TwinStrep-Flag-3CExpressionMammalianMutationPromoterCMVT (Enhanced CMV)Available sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only
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pCAS
Plasmid#206994PurposePlasmid expressing both SpCas9 and SaCas9DepositorUseTagsP2AExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BZLF1(Zta)
Plasmid#195787PurposeGateway Entry Clone of Epstein-Barr virus BZLF1DepositorInsertBZLF1 (BZLF1 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
opto-Cdc42
Plasmid#174510PurposeExpresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.DepositorInsertopto-Cdc42-mCherry
UseTags3xFLAG and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His hTFNG
Plasmid#67240PurposeExpresses N-His tagged nonglycosylated human serum transferrin in mammalian cellsDepositorInserthuman serum transferrin (TF Human)
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationAsn413 Asp, Asn611AspPromoterSV40Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RRV
Plasmid#188543PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editingDepositorInsertLbCas12a-RRV
UseCRISPR; Gateway compatible lbcas12a-rrv entry clo…TagsNLSExpressionPlantMutationG146R, D156R, R182VPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-PLXNB2
Plasmid#86236PurposePLXNB2 full-length human cDNA CDS in gateway entry plasmidDepositorInsertPLXNB2 (PLXNB2 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-2B-Tbx5
Plasmid#98619PurposeGateway entry vector TBX5DepositorInsertTBX5 (Tbx5 Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
NgOE FUNgGW
Plasmid#92231PurposeLentiviral expression of human NgDepositorInsertNg (NRGN Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CREB
Plasmid#68550PurposeAAV expression of CREBDepositorInsertCREB
UseAAVTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-mCREB
Plasmid#68551PurposeAAV expression of mCREBDepositorInsertmCREB
UseAAVTagsEGFPExpressionMammalianMutationS133APromoterCMVAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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neurod1:cre; cmlc2:EGFP
Plasmid#173480Purposecre is driven by -5kb neurod1 promoter. The vector contain a cmlc2:EGFP makerDepositorInsertCre
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A FKBP12F36V-OGT
Plasmid#154287PurposeEntry vector encoding FKBP12(F36V)-2xHA-OGT (dTAG fused-O-GlcNAc Transferase)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x HA and FKBP12(F36V)ExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4-DdCBE-right side TALE
Plasmid#157843Purposeexpresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4-DdCBE- left side TALE
Plasmid#157844Purposeexpresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cellsDepositorInsertSOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Left TALE-G1397-N-DddA11-mCherry
Plasmid#179682PurposeExpression of base editor in mammalian cells and for cell sortingDepositorInsertCOX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
UseTagsExpressionMammalianMutationS1330I A1341V N1342S E1370K T1380IPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-CLIP-tag_fast
Plasmid#167275PurposeOverexpression of the fast variant (E30R) of CLIP-tag in E. coliDepositorInsertCLIPf-tag
UseTagsHis x10ExpressionBacterialMutationE30RPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-L2
Plasmid#127352Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hACE2-LgBiT
Plasmid#215837PurposeExpresses hACE2 receptor with LgBiT tag in mammalian cellsDepositorInserthACE2-LgBiT (ACE2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only