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Plasmid#12454DepositorInsertUBP43 (Usp18 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-hGtACR2-EYFP
Plasmid#67877PurposeMammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backboneDepositorInserthGtACR2
UseLentiviralTagsEYFPExpressionMammalianMutationPromoterUbCAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-SpG-P2A-hMLH1dn
Plasmid#211550PurposePrime editing in mammalian cellsDepositorInsertPEmax-SpG-P2A-hMLH1dn
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PIK3R1
Plasmid#174192PurposeExpresses PIK3R1 in mammalian cellsDepositorInsertPIK3R1 (PIK3R1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
BiP-mCherry
Plasmid#62233PurposeHamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.DepositorInserthamster BiP-mCherry-KDEL
UseTagsmCherryExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQdCas9-sgempty
Plasmid#159523PurposeExpression of dCas9 driven by lux cassette from Vibrio fischeriDepositorInsertquorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
UseCRISPRTagsExpressionMutationPromoterquorum sensing promoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCiVSP(C363S)-SD64TF
Plasmid#80334PurposeExpresses Ciona VSP (without phosphatase activity) in Xenopus OocyteDepositorInsertCiona intestinalis voltage-sensing phosphatase (C363S) (vsp Ciona intestinalis)
UseXenopus oocyte expressionTagsExpressionMutationChanged Cysteine 363 to SerinePromoterSP6Available sinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pS2513·PHP
Plasmid#122590PurposeConstitutive expressed pHiorine2 for pH quan. in gram-negative cellsDepositorInsertphIorine2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPem7Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
punc503GFP
Plasmid#64021Purpose503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expressionDepositorInsertunc-45b: GFP (unc45b Zebrafish)
UseGatewayTagsExpressionMutationPromoter503-bp promoter of zebrafish unc-45bAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISYmu1_reRNA
Plasmid#205358PurposeBacterial expression of ISYmu1 TnpB and its reRNADepositorInsertISYmu1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PIK3R1
Plasmid#174191PurposeExpresses PIK3R1 in mammalian cellsDepositorInsertPIK3R1 (PIK3R1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-sfGFP
Plasmid#184226PurposeExpresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.DepositorInsertSpyTag-sfGFP
UseTagsHis6, SpyTag, and TEV protease cleavage siteExpressionBacterialMutationPromoterT5Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTet-tns
Plasmid#170628PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTnsABC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-Halo-10-PFN1
Plasmid#204222PurposeExpression of Halo-Profilin-1 in tissue culture cellsDepositorInsertPFN1 (PFN1 Human)
UseTagsHalo, TEV site, SGLRSRAQAS linkerExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagC wt
Plasmid#19304DepositorInsertRas-related GTP binding C (RRAGC Human)
UseTagsGST and HAExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagA wt
Plasmid#19298DepositorInsertRas-related GTP binding A (RRAGA Human)
UseTagsGST and HAExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDD282
Plasmid#66823PurposeGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertGFP-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized GFPExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagD wt
Plasmid#19307DepositorInsertRas-related GTP binding D (RRAGD Human)
UseTagsGST and HAExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
His6-PRC1
Plasmid#69111PurposeFor bacterial expression of recombinant human PRC1 tagged with His.DepositorInsertProtein Regulator of Cytokinesis 1 (PRC1 Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBCCP-GFP-NLS
Plasmid#173923PurposeFluorescent labeling of the nuclear envelopeDepositorInsertBiotin carboxyl carrier protein, AcGFP1
UseTags3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only