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Plasmid#208650PurposeMammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoterDepositorInsertKif2c (Kif2c Cricetulus griseus (Chinese hamster))
UseTagseGFPExpressionMammalianMutationS92A , S106A , S108A, S112A , S186A, H530A, R534A…PromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
HSPA8_pLX307
Plasmid#98343PurposeLentiviral expression of HSPA8DepositorInsertHSPA8 (HSPA8 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mouse Alpha2 delta-2 pMT2
Plasmid#58732Purposeexpression of mouse Alpha2 delta-2 in mammalian cellsDepositorInsertalpha2 delta-2 (Cacna2d2 Mouse)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G13D
Plasmid#111851PurposeExpresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G13D (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 13 to Aspartic AcidPromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaoA-RLuc8
Plasmid#140976PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaoA-RLuc8 (GNAO1 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOKMSOX17FNV
Plasmid#206382PurposeExpresses four genes (human OCT4, KLF4, c-MYC, SOX17FNV) in mammalian cells, for lentivirus generation.DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7B2Jnk3a2
Plasmid#19729DepositorUseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaoB-RLuc8
Plasmid#140977PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaoB-Rluc8 (GNAO1 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-MAFB-P2A-mCherry
Plasmid#203866PurposeTet-inducible lentiviral plasmid expressing MAFB-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertMAFB (MAFB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_Hyg_mTurquoise2_Atg4BC74A
Plasmid#161733PurposeDoxycycline-inducible expression of Atg4B(C74A) fused with mTurquoise2 to inhibit autophagyDepositorInsertAtg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
UseLentiviral; Destinatioin vector for gateway cloni…TagsmTurquoise2ExpressionMammalianMutation220-222 TGC (Cys) is changed to GCA (Ala)PromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti.pFOXA2.5'.GFP
Plasmid#59407Purposeexpresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during developmentDepositorInsertapprox. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterFOXA2Available sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_TCF3_FLI1
Plasmid#205934PurposeExpress mEGFP-tagged fusion protein, TCF3_FLI1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSin-EF2-DMD53-GFP
Plasmid#138353PurposeExpressing a GFP reporter cassette, whose GFP reading frame is disrupted by insertion of partial DMD exon 53 sequencesDepositorInsertDMD exon 53 partial sequence to disrupt GFP reading frame (DMD Human)
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterEF2Available sinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-IL18R1-COMP5AP-AviTag-9xHis
Plasmid#157313PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertIL18R1 (IL18R1 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Kir6.2 K+ channel scFv [N363/71]
Plasmid#190546PurposeMammalian Expression of Kir6.2 K+ channel scFV. Derived from hybridoma N363/71.DepositorInsertKir6.2 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj11 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC27A1_STOP
Plasmid#161294PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC27A1 (SLC27A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223_FAM116A_WT
Plasmid#81863PurposeGateway Donor vector containing FAM116A ,part of the Target Accelerator Plasmid Collection.DepositorInsertFAM116A (DENND6A Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEFL GAL4dbd-KLF4 159-329
Plasmid#140148PurposeGAL4-DNA binding domain fusion protein with amino acids 159 to 329 of human KLF4 (repressor domain)DepositorInsertKLF4 aa 159-329 (KLF4 Human)
UseTagsGAL4dbdExpressionMammalianMutationDeleted amino acids 1 to 158 and 330 to 479PromoterEF1aAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP
Plasmid#75126PurposeRetroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domainDepositorInsertKMT6 (EZH2 Human)
UseRetroviralTagsNoneExpressionMutationSET domain replaced with the SET domain of EZH1PromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Kir6.1 K+ channel scFv [N366/60]
Plasmid#190547PurposeMammalian Expression of Kir6.1 K+ channel scFV. Derived from hybridoma N366/60.DepositorInsertKir6.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj8 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only