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Plasmid#80904PurposeInjection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2DepositorInsertDendra2 (elavl3 Synthetic)
UseZebrafish expressionTagsExpressionMutationPromoterHuCAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
BII-ChiNW-ASCL2
Plasmid#133384PurposePiggybac vector for constitutive expressionDepositorInsertASCL2 (ASCL2 Human)
UseTagsExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3_NSnoFbox-vhhGFP4
Plasmid#35580DepositorInsertNSnoFbox-vhhGFP4 (slmb Fly)
UseTagsExpressionMammalianMutationF box domain of slmb deleted.PromoterAvailable sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
908H
Plasmid#160240PurposeTetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attBDepositorInsertsTet Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter
UseTagsT2A GFPExpressionInsectMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_VHH-GFP4::CD8::mCherry
Plasmid#163917PurposeExpression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancersDepositorInsertFusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-AlstR-IRES2-EGFP
Plasmid#14895DepositorInsertAlstR (AstA-R1 Fly)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D CA
Plasmid#52247PurposeDrosophila expression of YFP-Ras85D constitutively active mutantDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ61L; CA mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Med29 pBacPAK8
Plasmid#15369DepositorInsertMed29 (MED29 Human)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D WT
Plasmid#52242PurposeDrosophila expression of YFP-Ras85DDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D DN
Plasmid#52246PurposeDrosophila expression of YFP-Ras85D dominant negative mutantDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS17N; DN mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGC(2.1)-U6.3
Plasmid#170516PurposePCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFPDepositorInsertgRNAcore(2.1)-pU6.3
UsePcr template vector for amplifying grnacore(2.1)-…TagsExpressionMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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YFP Rab4DN
Plasmid#99019PurposeLocalization and visualization of the dominant negative form of Rab4DepositorInsertRab4 DN (Rab4 Synthetic, Fly)
UseTagsYFPExpressionMammalianMutationS22N Dominant negativePromoterCMVAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124434PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in DrosophilaDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1-MBP-DmBam_13-36_AG
Plasmid#148839PurposeMammalian Expression of DmBam_13-36DepositorInsertDmBam_13-36 (bam Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM627
Plasmid#112532PurposepZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
UseTagsExpressionBacterialMutationPromoterJ23107, PL(HrtO), and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-2_dCrk
Plasmid#131146PurposeExpresses GST::dCrk (based on crk isoform RA).DepositorInsertCrk (Crk Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only