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Plasmid#182208PurposeFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegyptiDepositorInsert3xP3-GFP
UseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Talin-N-22
Plasmid#57673PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertTalin
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pICSL80004
Plasmid#50325DepositorInsertCDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFTK051
Plasmid#171323PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteBFP fluorescent reporter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pI2-MTU-DO-HIS
Plasmid#160016PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Histidine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHS248
Plasmid#87354PurposeS867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET constructDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/C574S/S867C/N1054CPromoterT7Available sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
p8267 LentiCRISPR v2 hygro sgNT-2
Plasmid#193978PurposeExpression of spCas9 and non-targeting control sgRNADepositorInsertspCas9
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH77911
Plasmid#50342DepositorInsert3'UTR, polyadenylation signal/terminator, ags (A. tumefaciens)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH72400
Plasmid#50338DepositorInsert3'UTR, polyadenylation signal/terminator, Atug7 (A. tumefaciens)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Talin-N-22
Plasmid#57420PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertTalin
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pICH71421
Plasmid#51834PurposeDepositorInsertplant 3'UTRpolyadenylation signal/terminator, Tom H4
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-halo-HA-p2a-neoR
Plasmid#161977Purposehomologous donor template for endogenous taggingDepositorInsertGAlinker-HA-halo-HA-p2a-neoR
UseTagsExpressionMutationPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-puroR-p2a-HA-halo
Plasmid#161979Purposehomologous donor template for endogenous taggingDepositorInsertpuroR-p2a-HA-halo-GSlinker
UseTagsExpressionMutationPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-8xHis
Plasmid#101699Purpose8xHis in domestication vectorDepositorInsertAATG-[8xHis]-AGCC
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-HsSmg7_H
Plasmid#146476PurposeMammalian Expression of HsSmg7DepositorInsertHsSmg7 (SMG7 Human)
UseTagsExpressionMammalianMutationone non silent mutation G2560A (V854I) and three …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFW11-ffluc
Plasmid#128487PurposeAllelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imagingDepositorInsertfirefly luciferase gene
UseAllelic replacement vectorTagsspy0535 downstream flanking region and spy0535 up…ExpressionMutationPromotersyntheticAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Talin-18
Plasmid#57672PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertTalin
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pICH42211
Plasmid#50255DepositorInsertpromoter nos (A. tumefaciens)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
TR-GFP
Plasmid#80591PurposeExpresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertGFP
UseAAVTagsExpressionMammalianMutationPromoterCBAAvailable sinceSept. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -