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Showing: 261 - 280 of 3090 results

  1. pCgVchcrRNA

    Plasmid
    #203815
    Purpose
    Expression of CRISPR RNA, shuttle vector for Corynebacterium glutamicum / E. coli
    Depositor
    Sheng Yang
    Insert
    CRISPR(BsaI)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203815-plasmid-map-sequence-id-403361

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  2. pWS173

    Plasmid
    #90960
    Purpose
    Cas9 gap repair vector - KanR
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    90960-plasmid-map-sequence-id-261712

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  3. pEF005-sgRNA-shuffle-in

    Plasmid
    #104441
    Purpose
    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Insert
    sgRNA-transRNA transcription by U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104441-plasmid-map-sequence-id-201145

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  4. lentiCas9n(D10A)-Blast

    Plasmid
    #63593
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, nickase mutant
    Promoter
    EFS-NS
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63593-plasmid-map-sequence-id-181681

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  17. pDG330

    Plasmid
    #100898
    Purpose
    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100898-plasmid-map-sequence-id-195326

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  18. pDSAY-vasaCas9sv40

    Plasmid
    #173669
    Purpose
    Cas9 expression vector for Anopheles
    Depositor
    Eric Marois
    Insert
    Cas9 under control of vasa promoter and SV40 terminator
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    vasa
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    173669-plasmid-map-sequence-id-343662

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Showing: 261 - 280 of 3090 results