1. p426 25Q GPD

   Plasmid

   #1181

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 25Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. TAL3403

   Plasmid

   #47122

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   ZebrafishCommunity-apoeb-right (apoeb Zebrafish)
   

   Use

   T7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

2. TAL3402

   Plasmid

   #47121

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   ZebrafishCommunity-apoeb-left (apoeb Zebrafish)
   

   Use

   T7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

3. KHBD00712

   Plasmid

   #39741

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG18412 (ph-p Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3puro-EGFP-GAPDH-Tudor-R rich

   Plasmid

   #225478

   Purpose

   Express mEGFP-GAPDH-Tudor-R fusion

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   GAPDH fused with Tudor and R-rich regions of TDRD3 (GAPDH Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 3XFLAG_GAPDH in pCW57_tTA_Blast

   Plasmid

   #154211

   Purpose

   Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDH

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   GAPDH (GAPDH Human)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE repeats (dox off)

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. plenti hSyn GAPDH

   Plasmid

   #220918

   Purpose

   Neuronal specific expression of GAPDH

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   GAPDH (GAPDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLC-CUL4A WT-P2A-Puro

   Plasmid

   #124304

   Purpose

   Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   CUL4A (CUL4A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMH-SFB-DYRK1B

   Plasmid

   #101771

   Purpose

   Mammalian expression of DYRK1B fusion protein

   Depositor

   Michael Huen

   Article

   Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

   Insert

   DYRK1B / MIRK (DYRK1B Human)
   

   Use

   Tags

   S peptide-flag-streptavidin binding peptide (SFB)

   Expression

   Mammalian

   Mutation

   siRNA-resistant allele

   Promoter

   Available since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-mA1-BE3

   Plasmid

   #113421

   Purpose

   Expresses mA1-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   mA1-BE3 (Apobec1 S. pyogenes and Bacteriophage PBS2, Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSIN-Gapdh

    Plasmid

    #175938

    Purpose

    knockout mouse Gapdh

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Gapdh gRNA (Gapdh Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-ApoE4

    Plasmid

    #197148

    Purpose

    Expresses ApoE4 in Mammalian cell

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE4 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-ApoE3

    Plasmid

    #197147

    Purpose

    Expresses ApoE3 in Mammalian cell

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE3 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-ApoE2

    Plasmid

    #197146

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE2 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV4-ApoE4

    Plasmid

    #87087

    Purpose

    Expresses ApoE4 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E4 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV4-ApoE3

    Plasmid

    #87086

    Purpose

    Expresses ApoE3 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E3 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. H3K9me3 biosensor

    Plasmid

    #120802

    Purpose

    FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

    Plasmid

    #183951

    Purpose

    Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

    Depositor

    Eugene Yeo

    Article

    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19-hAPOE-R-loop

    Plasmid

    #134901

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    APOE promoter (APOE Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ABE7.10-F148A

    Plasmid

    #132946

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    ABE7.10(F148A)
    

    Use

    CRISPR; Base editor

    Tags

    Expression

    Mammalian

    Mutation

    ABE7.10(F148A)

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. H3S10ph biosensor

    Plasmid

    #120809

    Purpose

    FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Threonine 3, Threonine 6, and Threonine 11 are mu…

    Promoter

    CMV

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge