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Plasmid#1181DepositorInserthtt 25Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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pcDNA3-Casp3 C163A-myc
Plasmid#11814DepositorInsertCaspase 3 catalytic mutant (CASP3 Human)
UseTagsMycExpressionMammalianMutationC163APromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR
Plasmid#118154PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationSee depositor comments belowPromoterEF1a core and U6Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp8
Plasmid#11817DepositorInsertCaspase 8 (CASP8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
Plasmid#118156PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pScaffold-H1 donor
Plasmid#118152PurposePCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoterDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp3-myc
Plasmid#11813DepositorInsertCaspase 3 (CASP3 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp7 C186A-Flag
Plasmid#11816DepositorInsertCaspase 7 catalytic mutant (CASP7 Human)
UseTagsFlagExpressionMammalianMutationC186APromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp8 C360A
Plasmid#11818DepositorInsertCaspase 8 catalytic mutant (CASP8 Human)
UseTagsHAExpressionMammalianMutationC360APromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp7-Flag
Plasmid#11815DepositorInsertCaspase 7 (CASP7 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp2-Flag
Plasmid#11811DepositorInsertCaspase-2 (CASP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-T2A-HygR
Plasmid#118153PurposeSpCas9 expression vectorDepositorInserthSpCas9-T2A-HygR
UseCRISPRTags3XFLAGExpressionMammalianMutationPromoterCAG and U6Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp2 C303A-Flag
Plasmid#11812DepositorInsertCaspase-2 catalytic mutant (CASP2 Human)
UseTagsFLAGExpressionMammalianMutationC303APromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp2-Delta Card
Plasmid#11810DepositorInsertCaspase-2 Delta Card (CASP2 Human)
UseTagsHisExpressionBacterialMutationDelta CardPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1
Plasmid#118158PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1
Plasmid#118157PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDSDepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp9 C287A
Plasmid#11819DepositorInsertCaspase 9 catalytically incompetent mutant (CASP9 Human)
UseTagsExpressionMammalianMutationC287A, catalytically incompetent mutant.PromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
Plasmid#118155PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 OPTN TSS-guide4
Plasmid#118199PurposeCRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1
Plasmid#118162PurposeCRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only