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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11814 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaspase 3 catalytic mutant
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Alt nameCaspase 3
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SpeciesH. sapiens (human)
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Insert Size (bp)950
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MutationC163A
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Entrez GeneCASP3 (a.k.a. CPP32, CPP32B, SCA-1)
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Tag
/ Fusion Protein
- Myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-Casp3 C163A-myc was a gift from Guy Salvesen (Addgene plasmid # 11814 ; http://n2t.net/addgene:11814 ; RRID:Addgene_11814) -
For your References section:
Biochemical characteristics of caspases-3, -6, -7, and -8. Stennicke HR, Salvesen GS. J Biol Chem. 1997 Oct 10. 272(41):25719-23. 10.1074/jbc.272.41.25719 PubMed 9325297