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pMA_Auxiliary 2

Plasmid

#102710

Purpose

Provides the Middle-to-End linker 2 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 3

Plasmid

#102711

Purpose

Provides the Middle-to-End linker 3 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4A

Plasmid

#102712

Purpose

Provides the End-to-End linker 4A which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4Γ

Plasmid

#102714

Purpose

Provides the End-to-End linker 4Γ which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA_Auxiliary 4Δ

Plasmid

#102715

Purpose

Provides the End-to-End linker 4Δ which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pUdOm1.1

Plasmid

#210273

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm1.2

Plasmid

#210274

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUdOm1.3

Plasmid

#210275

Purpose

Empty backbone with ampicillin resistance for intermediate expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUdOm1.4

Plasmid

#210276

Purpose

Empty backbone with ampicillin resistance for low expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUdOm1.5

Plasmid

#210277

Purpose

Empty backbone with ampicillin resistance for low expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
iMbody_pSANG10-3F

Plasmid

#184496

Purpose

Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

Depositor

Marcel Dinger

Article

Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

Insert

iMbody

Use

Tags

3xFLAG, 6xHis, and pelB leader

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-tTA-ER-C3

Plasmid

#134568

Purpose

Expresses a YFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

Depositor

Susan Janicki

Article

Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

Insert

tTA-ER

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSEVA63-GG

Plasmid

#208015

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Use

Tags

Expression

Mutation

Promoter

14G

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-α5last

Plasmid

#160270

Purpose

Expresses a concatemeric construct of a α4:β2:α5 (2:2:1) nAChR

Depositor

Pierre-Jean Corringer

Article

Prevost et al Cell Mol Life Sci. 2020 May 29. pii: 10.

Insert

α5last concatemer

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSAC212

Plasmid

#187912

Purpose

psac212-crispey-bar-ade2KO

Depositor

Hunter Fraser

Article

Chen et al Cell Genom. 2023 Feb 28;3(4):100273. doi

Insert

ade2 KO CRISPEY cassette

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA23-GG

Plasmid

#208013

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Use

Tags

Expression

Mutation

Promoter

14G

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pACYC.Taq.1Amb

Plasmid

#102794

Purpose

Expresses Taq DNA polymerases with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Use

Tags

Expression

Bacterial

Mutation

W168Stop; *(see below)

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSEVA13-GG

Plasmid

#208002

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Use

Tags

Expression

Mutation

Promoter

14G

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBPGUw-HACK-G4>QF2

Plasmid

#80277

Purpose

GAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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T1ACR1_EYFP_pcDNA3.1

Plasmid

#161027

Purpose

Expresses strongly red-shifted T1ACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

T1ACR1

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge