1. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-a and hU6

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. VV220: CoChR(C108S)-eGFP in fck

   Plasmid

   #58512

   Purpose

   Expresses CoChR with the C108S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   CoChR(C108S)-eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 108 to Serine

   Promoter

   a-CamKII

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. p667-UBC-CLK4-V5-miniTurbo_IDG-K

   Plasmid

   #189913

   Purpose

   Gateway destination clone of CLK4 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CLK4 (CLK4 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDestTol2CG2-U6:gRNA

   Plasmid

   #63156

   Purpose

   Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Type

   Empty backbone

   Use

   CRISPR; Tol2 destination vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-Cas9-T2A-GFP

   Plasmid

   #63155

   Purpose

   Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Insert

   Cas9-T2A-GFP
   

   Use

   CRISPR; Tol2 middle entry vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mouse Metabolic Gene CRISPRa sgRNA library

   Pooled Library

   #187081

   Purpose

   Lentiviral CRISPRa library targeting mouse metabolic genes for activation of gene expression

   Depositor

   Kivanc Birsoy

   Article

   Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

   Expression

   Mammalian

   Species

   Mus musculus

   Use

   Lentiviral

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

6. Human Metabolic Gene CRISPRa sgRNA library

   Pooled Library

   #187080

   Purpose

   Lentiviral CRISPRa library targeting human metabolic genes for activation of gene expression

   Depositor

   Kivanc Birsoy

   Article

   Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   Lentiviral

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

7. sgGFP_3

   Plasmid

   #78165

   Purpose

   sgGFP

   Depositor

   William Hahn

   Article

   Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

   Insert

   GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

8. HK1 gRNA (BRDN0001145157)

   Plasmid

   #77643

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human HK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   HK1 (HK1, HOOK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. HK1 gRNA (BRDN0001147564)

   Plasmid

   #77644

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human HK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   HK1 (HK1, HOOK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HK1 gRNA (BRDN0001146994)

    Plasmid

    #77645

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human HK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    HK1 (HK1, HOOK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Human sgRNA library Brunello in lentiGuide-Puro

    Pooled Library

    #73178

    Purpose

    Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Has Service

    Lentiviral Prep

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

12. Lenti-human-TF-gRNA-puromycin

    Pooled Library

    #162275

    Purpose

    This is a gRNA library targeting on ~1600 human transcription factors for genetic screening.

    Depositor

    Chunliang Li

    Article

    Zhang et al Elife. 2020 Oct 1;9. pii: 57858. doi: 10

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

13. Mouse sgRNA library Brie in lentiGuide-Puro

    Pooled Library

    #73633

    Purpose

    Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Has Service

    Lentiviral Prep

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

14. Lenti-human-UBDUB-gRNA-puromycin-IRES-CFP

    Pooled Library

    #171531

    Purpose

    Pooled gRNA library against human ubiquitination and de-ubiquitination complex genes (UBDUB)

    Depositor

    Chunliang Li

    Article

    Wright et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

15. Human CRISPR Poison Exon Knockout Library

    Pooled Library

    #138084

    Purpose

    The human pgPoison library induces skipping of “poison” cassette exons and corresponding upstream constitutive exons by targeting 3' splice sites with paired gRNAs (pgRNAs).

    Depositor

    Robert Bradley

    Article

    Thomas et al Nat Genet. 2020 Jan;52(1):84-94. doi: 10

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

16. sgTP53_3

    Plasmid

    #78164

    Purpose

    sgTP53

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only