We narrowed to 2,848 results for: 52963

1. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1-a and hU6

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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1. IL13Ra1-NanoLuc BiBRET Vector

   Plasmid

   #238674

   Purpose

   Express IL13Ra1-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   IL13Ra1 (IL13RA1 Human)
   

   Tags

   NanoLuc (R)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Sept. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pDestTol2pA2-U6:gRNA

   Plasmid

   #63157

   Purpose

   Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Type

   Empty backbone

   Use

   CRISPR; Tol2 destination vector for gateway

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-Cas9

   Plasmid

   #63154

   Purpose

   Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Insert

   Cas9
   

   Use

   CRISPR; Tol2 middle entry vector for gateway

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. VV229: GCaMP6f in fck

   Plasmid

   #58514

   Purpose

   Expresses GCaMP6f under the a-CamKII promoter

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   GCaMP6f
   

   Use

   Lentiviral

   Tags

   C-terminal FCYENEV (ER export motif); this was no…

   Expression

   Mammalian

   Promoter

   a-CamKII

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck

   Plasmid

   #58524

   Purpose

   Expresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cells

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   sdChR(C138S, E154A)-TS-GCaMP6f-ER
   

   Use

   Lentiviral

   Tags

   GCaMP6f

   Expression

   Mammalian

   Mutation

   changed Cysteine 138 to Serine; changed Glutamate…

   Promoter

   a-CamKII

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. VV201: ChR2(C128S)-eGFP in fubi

   Plasmid

   #58510

   Purpose

   Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   ChR2(C128S)-eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Cysteine 128 to Serine

   Promoter

   Ubiquitin

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. VV221: sdChR(C138S)-TS-eGFP-ER in fck

   Plasmid

   #58513

   Purpose

   Expresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   sdChR(C138S)-TS-eGFP-ER
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 138 to Serine

   Promoter

   a-CamKII

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. VV228: sdChR(C138S E154A)-TS in fck

   Plasmid

   #58523

   Purpose

   Expresses sdChR(C138S E154A) in mammalian cells

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   sdChR(C138S, E154A)-TS
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   changed Cysteine 138 to Serine; changed Glutamate…

   Promoter

   a-CamKII

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. VV220: CoChR(C108S)-eGFP in fck

   Plasmid

   #58512

   Purpose

   Expresses CoChR with the C108S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   CoChR(C108S)-eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 108 to Serine

   Promoter

   a-CamKII

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. p667-UBC-CLK4-V5-miniTurbo_IDG-K

    Plasmid

    #189913

    Purpose

    Gateway destination clone of CLK4 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CLK4 (CLK4 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDestTol2CG2-U6:gRNA

    Plasmid

    #63156

    Purpose

    Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Type

    Empty backbone

    Use

    CRISPR; Tol2 destination vector for gateway

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLeGO.sgGata1.4.RUNX1A.iG2

    Plasmid

    #181976

    Purpose

    gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

    Depositor

    Jan-Henning Klusmann

    Inserts

    GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
    RUNX1A (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-AML1-ETO

    Plasmid

    #12428

    Depositor

    Scott Hiebert

    Article

    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

    Insert

    AML1-ETO fusion (RUNX1, RUNX1T1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV5-AML1B

    Plasmid

    #12426

    Purpose

    pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

    Depositor

    Scott Hiebert

    Article

    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

    Insert

    AML1B (RUNX1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. Runx1_pCSdest

    Plasmid

    #53802

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    Runx1 (RUNX1 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pME-Cas9-T2A-GFP

    Plasmid

    #63155

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUHD-AML1-ETO

    Plasmid

    #12429

    Depositor

    Dong-Er Zhang

    Article

    Rhoades et al Blood. 2000 Sep 15. 96(6):2108-15.

    Insert

    AML1-ETO (RUNX1, RUNX1T1 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pCMV5-AMLn-CAGEn-NES (Nrdj1)

    Plasmid

    #241408

    Purpose

    Proximity-triggered Protein Trans-Splicing to generate the splice product AML1-ETO, Figure 2 in Nature Chemical Biology 20.10 (2024): 1353-1360.

    Depositor

    Thomas Muir

    Article

    Lee et al bioRxiv [Preprint]. 2023 Jul 10:2023.07.

    Insert

    AMLn-CAGEn-NES (Nrdj1) (RUNX1 Human, Synthetic)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCV-RUNX1-4D

    Plasmid

    #238535

    Purpose

    Used for overexpression of human RUNX1 in mammalian cells. Contains RUNX1 phosphomimetic mutations.

    Depositor

    Diane Krause

    Article

    Kwon et al Blood. 2024 Oct 24;144(17):1800-1812. do

    Inserts

    RUNX1 (RUNX1 Human)
    Puromycin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S249D, S266D, T273D, S276D

    Promoter

    MSCV and PGK

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCV-RUNX1-WT

    Plasmid

    #238536

    Purpose

    Used as a positive control for overexpression of human RUNX1 in mammalian cells.

    Depositor

    Diane Krause

    Article

    Kwon et al Blood. 2024 Oct 24;144(17):1800-1812. do

    Inserts

    RUNX1 (RUNX1 Human)
    Puromycin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MSCV and PGK

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge