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Plasmid#111014PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertcell traversal protein for ookinetes and sporozoites (CelTOS) (PF3D7_1216600 )
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-mCerulean
Plasmid#66084PurposeG protein alpha-s internally tagged with mCerulean and EE epitopeDepositorInsertG-alpha-s-EE-mCerulean (Gnas Aequorea victoria, Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHD-dsRed-attP_dCrk_flankingHRs
Plasmid#131141PurposeContains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).DepositorUseCRISPR and Cre/Lox; Homology directed repair temp…TagsExpressionMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiR-CIP2A-stop-mut-miR-375-mutA-E
Plasmid#53714PurposeLuciferase reporter assay for CIP2A with mutated firefly stop codon that also has five miR-375 binding site mutationsDepositorInsertCIP2A (CIP2A Human)
UseLuciferaseTagsExpressionMutationMutation on the stop codon of firefly luciferase …PromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT3EF1aH-myr-Akt
Plasmid#179909PurposeThis plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs
Plasmid#126444PurposeExpresses a mutated version of mouse Fndc3b circular RNA with the miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutatedDepositorInsertFndc3b (Fndc3b Mouse)
UseTagsExpressionMammalianMutationcircFndc3b exons are separated by a miniature int…PromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
Plasmid#118155PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-SNAPf (w878-1)
Plasmid#29652DepositorTypeEmpty backboneUseEntry vectorTagsSNAPfExpressionMutationPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-CLIPf (w877-2)
Plasmid#29650DepositorTypeEmpty backboneUseEntry vectorTagsCLIPfExpressionMutationPromoterAvailable sinceJuly 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
BirA in pENTR1A (w860-1)
Plasmid#29645DepositorInsertBirA
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-HaloTag (w876-1)
Plasmid#29644DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseEntry vectorTagsHaloTagExpressionMutationPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-IVT
Plasmid#205145PurposeIVT template of AcGFP with MCS in frame and polyADepositorInsertAcGFP1
UseIn-vitro transcriptionTagsExpressionMutationPromoterT7Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-SB1-LUC (w832-1)
Plasmid#29647DepositorInsertFirefly Luciferase
UseEntry vectorTagsSB1ExpressionMutationPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-stop-IVT
Plasmid#205146PurposeIVT template of AcGFP stop codon between the ORF and MCS and polyADepositorInsertAcGFP1
UseIn-vitro transcriptionTagsExpressionMutationPromoterT7Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK BirA Hygro (w874-1)
Plasmid#29649DepositorInsertBirA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFFLuc-IVT
Plasmid#205147PurposeIVT template of FireFly luciferase stop codon between the ORF and MCS and polyADepositorInsertFireFly luciferase
UseLuciferase; In-vitro transcriptionTagsExpressionMutationPromoterAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
3' EGFP pXOON
Plasmid#48699PurposeCreates a 3' Fusion of EGFP to insert for expression in Xenopus oocytes or mammalian cellsDepositorTypeEmpty backboneUseXenopus oocyte expressionTagsEGFPExpressionMammalianMutationPromoterT7Available sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRLuc-IVT
Plasmid#205148PurposeIVT template of Renilla luciferase stop codon between the ORF and MCS and polyADepositorInsertRenilla Luciferase
UseLuciferase; In-vitro transcriptionTagsExpressionMutationPromoterT7Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1453
Plasmid#143279PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDLX2 (DLX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
371-A
Plasmid#97026PurposeyORFDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only