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Showing: 2701 - 2720 of 50971 results

  1. pGBW-m4134354

    Plasmid
    #152241
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152241-plasmid-map-sequence-id-300274

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  2. pGBW-m4134104

    Plasmid
    #152061
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152061-plasmid-map-sequence-id-308749

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  3. pGBW-m4133153

    Plasmid
    #151998
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;S-tag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    151998-plasmid-map-sequence-id-308759

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  4. pGBW-m4133248

    Plasmid
    #152036
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 endoRNAse
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 endoRNAse (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;TapTag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152036-plasmid-map-sequence-id-308743

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  5. pGBW-m4046627

    Plasmid
    #153916
    Purpose
    Yeast expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153916-plasmid-map-sequence-id-300484

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  6. pGBW-m4134257

    Plasmid
    #152053
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 endoRNAse
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 endoRNAse (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 19, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152053-plasmid-map-sequence-id-297882

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  7. pFOXO4-donor

    Plasmid
    #112312
    Purpose
    CRISPR donor plasmid to tag human transcription factor FOXO4 with GFP
    Depositor
    Kevin White
    Insert
    FOXO4 homology arms flanking EGFP-IRES-Neo cassette (FOXO4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112312-plasmid-map-sequence-id-228464

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  8. KCTD13A

    Plasmid
    #74654
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KCTD13A (KCTD13 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pUC57-Homology Arms-BRD4-HaloTag

    Plasmid
    #228575
    Purpose
    CRISPR donor plasmid to tag human BRD4 with Halo tag; for CRISPR-mediated gene insertion
    Depositor
    Marcin Imielinski
    Insert
    Homology Arms-BRD4-HaloTag (BRD4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    228575-plasmid-map-sequence-id-461799

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  10. AAV-hSyn-PiGM-Ii (149, 535/81, eGFP)

    Plasmid
    #221610
    Purpose
    AAV encoding the 149 amino acid version PiGM-Ii with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).
    Depositor
    John Lin
    Insert
    PiGM-Ii (149, eGFP)
    Use
    AAV
    Tags
    Expression
    Mutation
    RGS10(truncation after amino acid 149)
    Promoter
    Human Synapsin
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    221610-plasmid-map-sequence-id-461233

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  12. pBB328

    Plasmid
    #221533
    Purpose
    Plasmid for the introduction of Mariner transposon that inserts deoxyviolacein cassette behind indigenous promoters in target bacterial strain. Plasmid has tetracycline marker for selection.
    Depositor
    Brett Barney
    Inserts
    vioABCE
    Mariner transposase and transposon backbone from pBB298 (originally from pEB001)
    Tetracycline antibiotic cassette from pRK415
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None, contains RBS site
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    221533-plasmid-map-sequence-id-442457

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  13. LRP1A-c004

    Plasmid
    #205112
    Purpose
    LRP1 protein expression
    Depositor
    Paul Brennan
    Insert
    LRP1 (LRP1 Human)
    Use
    Tags
    AviTag and GP64sp-6His-TEV
    Expression
    Insect
    Mutation
    WT
    Promoter
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205112-plasmid-map-sequence-id-412366

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  14. mCerulean3-Bik-s2193

    Plasmid
    #177425
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bik, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    Human ferritin
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177425-plasmid-map-sequence-id-347947

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  15. pDEST27-GST-hSFRP1(aa181-314)

    Plasmid
    #189548
    Purpose
    Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.
    Depositor
    Haian Fu
    Insert
    truncated secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa181-314
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189548-plasmid-map-sequence-id-374132

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  16. pDEST27-GST-hSFRP1(aa53-169)

    Plasmid
    #189545
    Purpose
    Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.
    Depositor
    Haian Fu
    Insert
    truncated secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa53-169
    Promoter
    Available since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    189545-plasmid-map-sequence-id-374145

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  17. pT22iD36CriT(#388)

    Plasmid
    #184062
    Purpose
    cyclofen-inducible CRE activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    CRE-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    Expression
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Zf-Dio3
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184062-plasmid-map-sequence-id-364011

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  18. GLuc VLP

    Plasmid
    #182213
    Purpose
    YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Gaussia luciferase tagged with VP2C (GAL10 promoter).
    Depositor
    Claudia Vickers
    Inserts
    VP2C-GLuc
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    March 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182213-plasmid-map-sequence-id-357719

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  19. CNN3A-c007

    Plasmid
    #175457
    Purpose
    Protein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.
    Depositor
    Opher Gileadi
    Insert
    CNN3 (CNN3 Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175457-plasmid-map-sequence-id-353125

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  20. pGBW-m4133156

    Plasmid
    #152316
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;S-tag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    Sept. 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152316-plasmid-map-sequence-id-313290

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Showing: 2701 - 2720 of 50971 results