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Plasmid#78116PurposeNt His-tag Mustela putorius furo Sacsin 94-1381DepositorInsertSacsin Mustela putorius furo 94-1381
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro p21 K161Q, K163Q
Plasmid#78792PurposeRetroviral expression of p21 K161Q, K163QDepositorInsertP21 (CDKN1A Human)
UseRetroviralTagsExpressionMammalianMutationK161Q, K163Q muationPromoter5'LTRAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-RIPK3 2KR(89,501)
Plasmid#78832Purposeretroviral expression of RIPK3 2KR(89,501)DepositorInsertRIPK3 (RIPK3 Human)
UseRetroviralTagsExpressionMammalianMutationK89R, K501RPromoter5'LTRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-RIPK3 2KR(55,363)
Plasmid#78831Purposeretroviral expression of RIPK3 2KR(55,363)DepositorInsertRIPK3 (RIPK3 Human)
UseRetroviralTagsExpressionMammalianMutationK55R, K363RPromoter5'LTRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro p21 K161R, K163R
Plasmid#78791PurposeRetroviral expression of p21 K161R, K163RDepositorInsertp21 (CDKN1A Human)
UseRetroviralTagsExpressionMammalianMutationK161R, K163R_muationPromoter5'LTRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV/TO-Puro-FLAG-hDIXDC1 WT
Plasmid#61218PurposeLentivirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterCMV/TOAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Delta-ATG1-mMCL1
Plasmid#45823PurposeThis plasmid was made to show that the two MCL-1 bands DO NOT arise from a non-canonical translation initiation downstream of the start codon.DepositorInsertDelta ATG1-mMCL1
UseTagsExpressionMammalianMutationThe first amino acid (ATG) is deleted from the fu…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-mMCL1 Delta C22
Plasmid#45820DepositorInsertmMCL-1 Delta C22
UseTagsExpressionMammalianMutationC-terminal 22 amino acids are deleted from full l…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Delta N67-mMCL1
Plasmid#45819DepositorInsertDelta N67-mMCL-1
UseTagsExpressionMammalianMutationN-terminus 67 amino acids deleted from full lengt…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-THAP11-rescue-3xFLAG
Plasmid#36998DepositorInsertTHAP11 (THAP11 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationMutations were generated at codons 273-275 conver…PromoterAvailable sinceMay 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
integrin beta6 Y762F pBabe puro
Plasmid#13597DepositorInsertintegrin beta 6 Y762F (ITGB6 Human)
UseRetroviralTagsExpressionMammalianMutationY762FPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE
Plasmid#85452PurposeLentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.DepositorInsertU6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai
Plasmid#202571PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Methionine 91 to Alanine, ORF1p Glu…PromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai
Plasmid#202568PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…PromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai
Plasmid#202569PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Arginine 261 to AlaninePromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cas9-T2A-EGFP-ires-puro
Plasmid#78311PurposeExpresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.DepositorInsertWT SpCas9-T2A-EGFP-ires-puromycin resistance
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterCAGAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin
Plasmid#134860PurposeExpresses EGFP-tagged recycling endosome markerDepositorInsertRAB11B (RAB11B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato-NLS. PGK-puro
Plasmid#136261PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomato-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A iRFP670 P2A puro
Plasmid#122182PurposeThe plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsUseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only