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Plasmid#118396Purposeanalysis of cis-splicing by mini inteinsDepositorInsertMjaTFIIB mini intein
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBHDuet45
Plasmid#118393Purposeanalysis of cis-splicing by mini inteinsDepositorInsertMjaTFIIB mini intein
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBHDuet173
Plasmid#118398Purposeanalysis of cis-splicing by mini inteinsDepositorInsertMvuTFIIB mini intein
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPS3911
Plasmid#113330Purposecontrol for Hermes transposition; TIR-flanked LEU2, transposase absentDepositorInsertbeta-isopropyl malate dehydrogenase
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-E228A-PvuRts1I
Plasmid#86896Purposeexpress E228A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationE228APromoterT7Available sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-W215A-PvuRts1I
Plasmid#86898Purposeexpress W215A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationW215APromoterT7Available sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PvuRts1I
Plasmid#86888Purposeexpress C-term His tagged PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsC-term His tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-E71A-PvuRts1I
Plasmid#86895Purposeexpress E71A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationE71APromoterT7Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-D57A-PvuRts1I
Plasmid#86892Purposeexpress D57A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationD57APromoterT7Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-R208A-PvuRts1I
Plasmid#86891Purposeexpress R208A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationR208APromoterT7Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-E20A-PvuRts1I
Plasmid#86897Purposeexpress E20A variant PvuRts1I in ER2566 strainDepositorInsertPvurts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationE20APromoterT7Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-D70A-PvuRts1I
Plasmid#86890Purposeexpress D70A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationD70APromoterT7Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pST50Trc4-HPCNDHFR
Plasmid#63998PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-HPCNDHFR
Plasmid#63962PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-HPCNDHFR
Plasmid#63980PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-FLAGHISNDHFR
Plasmid#63983PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & FLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-HPCNDHFR
Plasmid#63944PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
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