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Showing: 2621 - 2640 of 16483 results

  1. pHSN401

    Plasmid
    #50588
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    maize codon optimized
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50588-plasmid-map-sequence-id-75660

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  2. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

    Plasmid
    #160736
    Purpose
    Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    160736-plasmid-map-sequence-id-326992

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  5. p2R3a-nosT

    Plasmid
    #71272
    Purpose
    Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    nopaline synthase terminator
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71272-plasmid-map-sequence-id-129823

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  6. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

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  8. pBUN411

    Plasmid
    #50581
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    maize codon optimized
    Promoter
    OsU3p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50581-plasmid-map-sequence-id-311045

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  10. pAC8_MF-p10-mCh (VE5626)

    Plasmid
    #139772
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with mCherry cDNA under p10 to monitor infection.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    mCherry fluorescent protein
    Use
    Tags
    Expression
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139772-plasmid-map-sequence-id-324177

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  11. p2R3a-3AT

    Plasmid
    #71273
    Purpose
    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71273-plasmid-map-sequence-id-129830

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  13. pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST

    Plasmid
    #109048
    Purpose
    Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vector
    Depositor
    Hillel Adesnik
    Article
    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/
    Has Service
    AAV9
    Insert
    eGtACR1-ST
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    July 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    109048-plasmid-map-sequence-id-264817

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  17. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

    Plasmid
    #140228
    Purpose
    RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.
    Depositor
    Stanley Qi
    Article
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    Use
    Lentiviral
    Tags
    BFP
    Expression
    Mammalian
    Mutation
    Promoter
    RRM2 endogenous promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140228-plasmid-map-sequence-id-291990

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  18. pSUC2::GNSI (NrsR)

    Plasmid
    #121537
    Purpose
    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    Use
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Promoter
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121537-plasmid-map-sequence-id-235299

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  19. pGLTR-X-GFP

    Plasmid
    #58245
    Purpose
    GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFP
    Depositor
    Stephan Geley
    Article
    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:
    Inserts
    attR1-Cm(R)-CcdB-attR2
    TetR-NLS-T2A-GFP
    Use
    Lentiviral and RNAi; Gateway destionation vector
    Tags
    GFP, NLS, T2A, and TetR
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and no
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58245-plasmid-map-sequence-id-89669

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  20. pCYC1pr-GNSI (LEU)

    Plasmid
    #111450
    Purpose
    GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    Use
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Promoter
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    111450-plasmid-map-sequence-id-211605

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Showing: 2621 - 2640 of 16483 results