Addgene

We narrowed to 34,877 results for: kan

Showing: 2621 - 2640 of 34877 results

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VCDstubΔ741-752-GFP

Plasmid

#33123

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains a signal sequence fused to a very small …

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
PB-TO-hNGN2

Plasmid

#172115

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

hNGN2 (NEUROG2 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Mutation

Promoter

EF1a and TRE3G

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PG13-mCherry-EF1a-PuroR-EGFP

Plasmid

#195292

Purpose

piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

mCherry
PuroR-P2A-EGFP

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSynI-V5-TurboID(N)-GPI

Plasmid

#163696

Purpose

Expresses n-terminal half of TurboID on surface of neurons

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-VARNAM2

Plasmid

#195520

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

Synapsin

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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hHSF4b WT

Plasmid

#118356

Purpose

This plasmid expresses human HSF4b WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hHSF4b (HSF4 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1 T2 CRIPR in pX330

Plasmid

#72833

Purpose

CRISPR/Cas to target the AAVS1 locus in human cells

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Cas9 and gRNA for targeting the AAVS1 locus in human cells

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

Plasmid

#166055

Purpose

Expresses the full length TurboID on the surface of astrocytes

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

Plasmid

#163697

Purpose

Expresses c-terminal half of TurboID on surface of astrocytes

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMK287 (mAID-Hygro)

Plasmid

#72825

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Hygro

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221_EGFP

Plasmid

#25899

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

EGFP

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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Dual opposing T7 promoters reporter plasmid

Plasmid

#156457

Purpose

A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Insert

NeoR/KanR

Use

Tags

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSCM001

Plasmid

#124605

Purpose

Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

Depositor

David Summers

Article

Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

Insert

mCherry-pHluorin

Use

Tags

mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

Expression

Bacterial

Mutation

Promoter

pDAB

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSecTag-ATX

Plasmid

#17839

Depositor

Steven Rosen

Article

Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

Insert

Autotaxin (ENPP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pSecTag-T210A-ATX

Plasmid

#17840

Depositor

Steven Rosen

Article

Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

Insert

T210A-ATX (ENPP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pMK286 (mAID-NeoR)

Plasmid

#72824

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Neo

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRT498-OtUBD

Plasmid

#190089

Purpose

Expresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJY-RpABE

Plasmid

#112872

Purpose

binary vector for ABE expression in Arabidopsis

Depositor

Jin-Soo Kim, Jae-Young Yun

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA cassette

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMK281 (mCherry2-Hygro)

Plasmid

#72797

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Hygro

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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hMEF2A WT

Plasmid

#118354

Purpose

This plasmid expresses human MEF2A-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hMEF2A (MEF2A Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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