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Addgene
Showing: 2581 - 2600 of 3786 results
  1. mFzd4CRDL(42-203)-6xHis_pAcGP67a

    Plasmid
    #216379
    Purpose
    Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells
    Depositor
    Insert
    Frizzled-4 (Fzd4 Mouse)
    Use
    Baculovirus
    Tags
    6xHis and GP64 signal sequence
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Availability
    Academic Institutions and Nonprofits only
  2. pPbU6_hdhfr/yfcu

    Plasmid
    #216422
    Purpose
    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR; Expression in plasmodium berghei
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pPbU6_hdhfr/yfcu_Cas9

    Plasmid
    #216423
    Purpose
    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR; Expression in plasmodium berghei
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pCMV-tadDE-PcrA M6-UGI

    Plasmid
    #221236
    Purpose
    Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.
    Depositor
    Insert
    tadDE-PcrA M6-UGI
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pAAV-CMV-dSa VP64 Hbb

    Plasmid
    #99693
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    Insert
    dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pLV-REPORT(EF1a)-TTN

    Plasmid
    #172327
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-alpha
    Availability
    Academic Institutions and Nonprofits only
  7. pLV-REPORT(PGK)

    Plasmid
    #172328
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Availability
    Academic Institutions and Nonprofits only
Showing: 2581 - 2600 of 3786 results