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Plasmid#236122PurposePlasmid encoding Gal4-NZF transcription factor with GS linkers between the component domains of NZF, under control of CMV promoter with two TetR binding sitesDepositorInsertGal4-NZF (GS Linkers)
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW216 PGK-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)
Plasmid#236131PurposePlasmid encoding the ZNF250-derived portion of the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoterDepositorInsertZNF250(1-4)-deImmunLink-NZF
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW171 CMV-TO-UTRN-43ZF-VP64 (FLP-IN)
Plasmid#236161PurposePlasmid encoding the UTRN-43 zinc finger array attached by a GS linker to the VP64 transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertUTRN-43ZF-VP64
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW446 CMV-TO-ADAR2dd(E488Q, T501A) (FLP-IN)
Plasmid#236125PurposePlasmid encoding free ADAR2dd under control of CMV promoter with two TetR binding sitesDepositorInsertADAR2dd (ADARB1 Human, Synthetic)
UseTagsExpressionMammalianMutationE488Q, T501APromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW418 PGK-IKZF1(2-3)-realDeimmunLink-NZF (FLP-IN)
Plasmid#236155PurposePlasmid encoding the IKZF1-derived portion of the ZNF35/IKZF1 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoterDepositorInsertIKZF1(2-3)-deimmunLink-NZF
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW368 CMV-TO-UtroUpZF-deImmunLink-NZF(FLP-IN)
Plasmid#236154PurposePlasmid encoding the UtroUp zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertUtroUpZF-deImmunLink-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Esyn-FlpX-TVA950-EYFP-WPRE
Plasmid#234710PurposepAAV transfer plasmid with enhanced Synapsin promoter driving Flp-dependent expression of TVA950-eYFPDepositorInsertTVA950-eYFP
UseAAV; Has fdio cassette for flp-dependent expressi…TagsExpressionMammalianMutationPromoterenhanced SynapsinAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP1633 - pAAV-AiE2114m-minBG-FlpO-HGHpA
Plasmid#230678PurposeAiE2114m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsInsertFlpO
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP1537 - pAAV-AiE2135m_3xC2-minBG-FlpO-HGHpA
Plasmid#220709PurposeAiE2135m_3xC2 is an optimized enhancer sequence, designed to induce Flp-dependent recombination in specific populations of brain cellsInsertFlpO
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
EK0209 CMV-TO-mCherry-SG(s70587)SG-EGFP (FLP-IN)
Plasmid#191174PurposeMammalian expression of EK0285 with CMV-tetO promoter instead of SFFV.DepositorInsertCMV-TO:mCherry:s70587:EGFP
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0344 SFFV-mCherry-SG(Bdnf.s1)SG-Cre (FLP-IN)
Plasmid#191158PurposeExpression of a sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, Cre output; 90 bp longDepositorInsertmCherry:Bdnf.s1:Cre
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)
Plasmid#191161PurposeExpression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cellsDepositorInsertEGFP.t2
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0341 SFFV-mCherry-SG(Bdnf.s1L153)SG-EGFP (FLP-IN)
Plasmid#191150PurposeExpression of a 153 bp long sensor for murine Bdnf 3' UTR in mammalian cellsDepositorInsertmCherry:Bdnf.s1L153:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0358 SFFV-mCherry-SG(Bdnf.s1L36)SG-EGFP (FLP-IN)
Plasmid#191152PurposeExpression of a 36 bp long sensor for murine Bdnf 3' UTR in mammalian cellsDepositorInsertmCherry:Bdnf.s1L36:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP949-Slc17a7-IRES2-FlpO-WPRE-neo
Plasmid#181776Purposetargeting vectorDepositorInsertSlc17a7 homology arms, FlpO
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP937-Gpr139-IRES2-FlpO-WPRE-neo
Plasmid#181768Purposetargeting vectorDepositorInsertGpr139 homology arms, FlpO
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP936-Chrna6-IRES2-FlpO-WPRE-neo
Plasmid#181766Purposetargeting vectorDepositorInsertChrna6 homology arms, FlpO
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)
Plasmid#191164PurposeSimilar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequencesDepositorInsertmCherry:s70587:EGFP (additional cutsites)
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR
Plasmid#68347PurposeAAV plasmid expressing the FlpO recombinase. with empty gRNADepositorInsertFlPO
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)
Plasmid#191159PurposeExpression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry outputDepositorInsertmTagBFP:EGFP.s2:mCherry
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA
Plasmid#108833PurposeExpresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDsDepositorInsertFlp recombinase fragment aa28 to aa396
UseCre/Lox and Synthetic BiologyTagsABI,NLS and GAIExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
EK0423 SFFV-mTagBFP2-SGc(s70587)cSG-mCherry (FLP-IN)
Plasmid#191162PurposeExpression of a sensor for synthetic sequence 1 (EK0208) in mammalian cells; mTagBFP2 marker, mCherry outputDepositorInsertmTagBFP:s70587:mCherry
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
Plasmid#159810PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO
Plasmid#68405PurposeThe prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoterDepositorInserts3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
FlpO
UseCRISPRTagsmTurquoise2 (BFP)ExpressionMammalianMutationPromoterSynthetic Probasin ARRx2 and U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
EK0387 SFFV-mTagBFP2-SGc(Bdnf.s1)cSG-mCherry (FLP-IN)
Plasmid#191157PurposeExpression of a sensor for murine Bdnf 3' UTR in mammalian cells; mTagBFP2 marker, mCherry output; 90 bp longDepositorInsertmTagBFP2:Bdnf.s1:mCherry
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP1635 - pAAV-AiE2116m-minBG-DIO-FlpO-WPRE-BGHpA
Plasmid#230680PurposeAiE2116m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsInsertFlpO
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
YH001 CMV-TO-tm-GS+XTEN-ADAR2(DD-E396A)-RXR (FLP-IN)
Plasmid#233383PurposeER-retained catalytically inactive ADAR2dd (E396A mutation)DepositorInsertTM-ADAR2dd
UseTagsExpressionMammalianMutationE396APromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-TeNT-P2A-EGFP
Plasmid#176282PurposeViral vector for co-expression of TeNT and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertTeNT-P2A-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLPX-rc [Jaws-KGC-tdTomato-ER2]
Plasmid#195488PurposeAAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in FLPX/reversed (Frt-dependent) manner.DepositorInsertJaws-KGC-tdTomato-ER2
UseAAVTagsER2, KGC, and tdTomatoExpressionMammalianMutationK200R W214FPromoterCAGAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP
Plasmid#176280PurposeViral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertmNaChBac-P2A-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBW2156_pCAG-PV1-FlpO-N27-L1-GID1-NLS-BGHpA
Plasmid#108747PurposeExpresses N-terminus to aa27 of Flp recombinase fused to GID1 CIDDepositorInsertFlp recombinase fragment N-terminus to aa27
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-mNaChBacMUT-P2A-EGFP
Plasmid#176281PurposeViral vector for co-expression of non-functional NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertmNaChBacMUT-P2A-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationE191KPromoterhuman Synapsin IAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA
Plasmid#108748PurposeExpresses aa28 to C-terminus of Flp recombinase fused to GAI CIDDepositorInsertFlp recombinase fragment aa28 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
AiP978-pAAV-mscRE4-minBGpromoter-FlpO-WPRE-hGHpA
Plasmid#163472PurposeDirect-expressing FlpO AAV Virus. Alias: AiP978 - pAAV-AiE2004m-minBG-FlpO-WPRE-HGHpAInsertFlpO
UseAAVTagsExpressionMutationPromoterBeta Globin minimal promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-Kir2.1-P2A-EGFP
Plasmid#176278PurposeViral vector for co-expression of Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertKir2.1-P2A-EGFP (Kcnj2 Mouse, Synthetic)
UseAAV and Cre/LoxTagsMycExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-mCherry-P2A-FlpO
Plasmid#73471PurposeExpresses mCherry and FlpO for monosynaptic circuit mapping and genetic manipulationDepositorInsertmCherry-P2A-FlpO
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)
Plasmid#191145PurposeExpression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.DepositorInsert3UTR(Bdnf)
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP1038-pAAV-mscRE16-minBGpromoter-FlpO-WPRE-hGHpA
Plasmid#163475PurposeDirect-expressing FlpO AAV Virus. Alias: AiP1038-pAAV-mscRE16-minBGpromoter-FlpO-WPRE-hGHpAInsertFlpO
UseAAVTagsExpressionMutationPromoterBeta Globin minimal promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA
Plasmid#108770PurposeExpresses aa397 to C-terminus of Flp recombinase fused to PYL CIDDepositorInsertFlp recombinase fragment aa397 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry
Plasmid#176277PurposeViral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.DepositorInsertMCS-P2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only