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Plasmid#146071PurposeInsect Expression of DmTral-LSmR21EE23KR42EF44ADepositorInsertDmTral-LSmR21EE23KR42EF44A (tral Fly)
UseTagsExpressionInsectMutationR21E, E23K, R42E, F44A. Additionally, one silent …PromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-R42EF44A_C
Plasmid#146061PurposeInsect Expression of DmTral-R42EF44ADepositorInsertDmTral-R42EF44A (tral Fly)
UseTagsExpressionInsectMutationR42E, F44A. Additionally, two silent mutations A1…PromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C
Plasmid#146049PurposeInsect Expression of DmTral-LSmR21EE23KS13AI15ADepositorInsertDmTral-LSmR21EE23KS13AI15A (tral Fly)
UseTagsExpressionInsectMutationS13A, I15A, R21E, E23K, one silent mutation A198C…PromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C
Plasmid#146039PurposeInsect Expression of DmTral-FDF-ADADepositorInsertDmTral-FDF-ADA (tral Fly)
UseTagsExpressionInsectMutationFDF to ADA (407-409) mutation. Additionally, thre…PromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
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lenti MS2-P65-HSF1_Hygro
Plasmid#61426Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2DepositorInsertMS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
RP1B FUS 1-163 S12xQ
Plasmid#127193PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 S12xQ (FUS Human)
UseTagshexahistidineExpressionBacterialMutationS12xQ: S30Q, S44Q, S48Q, S53Q, S70Q, S84Q, S89Q, …PromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-XRCC1-gRNA res-Neo
Plasmid#176149PurposeXRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassetteDepositorInsertX-ray repair cross complementing 1 (XRCC1 Human)
UseLentiviralTagsExpressionMammalianMutationDual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…PromoterCMVAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS
Plasmid#85821PurposeiDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertZf Ubi::cMyc oDam_f_GFPoNLS
UseTol2 based vectorTagscMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…ExpressionMutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4B, residues 156-224, no Cys
Plasmid#180641PurposeBacterial expression for CHMP4B C-terminal tail, residues 156-224. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-140DepositorInsertCHMP4B (CHMP4B Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 156-224PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169_X
Plasmid#147983PurposeInsect Expression of DmNot1_1-1717-del1156-1169DepositorInsertDmNot1_1-1717-del1156-1169 (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A_X
Plasmid#147984PurposeInsect Expression of DmNot1_1-1717-del1156-1169-R1197AN1203AF1204ADepositorInsertDmNot1_1-1717-del1156-1169-R1197AN1203AF1204A (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA_X
Plasmid#147985PurposeInsect Expression of DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEADepositorInsertDmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres_X
Plasmid#147990PurposeInsect Expression of DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAresDepositorInsertDmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation8 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1-del1156-1169-dsRNAres_X
Plasmid#147991PurposeInsect Expression of DmNot1-del1156-1169-dsRNAresDepositorInsertDmNot1-del1156-1169-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W
Plasmid#147875PurposeInsect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAresDepositorInsertDmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W
Plasmid#147859PurposeInsect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAresDepositorInsertDmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_1-718-del280-310_AI
Plasmid#148949PurposeMammalian Expression of HsGIGYF2_1-718-del280-310DepositorInsertHsGIGYF2_1-718-del280-310 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G1-L640AV646A_AD
Plasmid#148520PurposeMammalian Expression of HseIF4G1-L640AV646ADepositorInsertHseIF4G1-L640AV646A (EIF4G1 Human)
UseTagsExpressionMammalianMutationone non silent mutation M432V (natural variant is…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only