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Plasmid#198388PurposeGCN1 expressionDepositorInsertGCN1 (GCN1 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZLCv2-3xFLAG-dCas9-HA-2xNLS
Plasmid#106357PurposeEmpty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitationDepositorTypeEmpty backboneUseCRISPR and LentiviralTags3XFLAG and HAExpressionMammalianMutationPromoterU6Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-SIRT6-H133Y-FLAG
Plasmid#102324PurposeHuman SIRT6 H133Y with FLAG tag for mammalian expressionDepositorInsertSIRT6 (SIRT6 Human)
UseTagsFLAGExpressionMammalianMutationchanged histidine 133 to tyrosinePromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5 CASTOR1-Flag Cterm
Plasmid#84488Purposeoverexpression of CASTOR1 with Flag tagDepositorInsertCASTOR1 (GATSL3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin2-L261A
Plasmid#73671PurposeoverexpressionDepositorInsertSesn2 (SESN2 Human)
UseTagsFLAGExpressionMammalianMutationL261A changed Leucine 261 to AlaninePromoterCMVAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-SIRT6-G60A-FLAG
Plasmid#102323PurposeHuman SIRT6 G60A with FLAG tag for mammalian expressionDepositorInsertSIRT6 (SIRT6 Human)
UseTagsFLAGExpressionMammalianMutationchanged glycine 60 to alaninePromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Flag CASTOR1 I280A
Plasmid#84495Purposeoverexpression of CASTOR1 I280A mutantDepositorInsertCASTOR1 I280A (GATSL3 Human)
UseTagsFLAGExpressionMammalianMutationI280APromoterCMVAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin2-E451A
Plasmid#73673PurposeoverexpressionDepositorInsertSesn2 (SESN2 Human)
UseTagsFLAGExpressionMammalianMutationE451A changed Glutamate 451 to AlaninePromoterCMVAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-SNAP
Plasmid#175295PurposeTrack mature FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with SNAP-tag
UseTagsSNAP-tagExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MO70-N-Flag-hCRACR2A-isoform-a
Plasmid#79592PurposeTransient and retroviral expressionDepositorInsertCRACR2A-isoform-a (CRACR2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-FUS/TLS-FLAGC
Plasmid#60362PurposePlasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.DepositorInsertFUS (FUS Human)
UseTagsFLAG and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-V600E
Plasmid#131723PurposeMammalian Expression of Flag tagged BRAFDepositorInsertBRAF (BRAF Human)
UseTags3xFLAGExpressionMammalianMutationV600EPromoterCMVAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-H3.3K27M-FLAG-BFP
Plasmid#209434PurposeLentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-K27M with BFPDepositorInsertH3.3-K27M
UseTagsExpressionMammalianMutationK27MPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-TAZ-N-Flag (S89A)
Plasmid#19026DepositorInsertTAZ (Wwtr1 Mouse)
UseTagsFlagExpressionMammalianMutationVector has no SV40 origin, S89A (site directed mu…PromoterAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA-Ago2-Y529E
Plasmid#215842PurposeExpression of AGO2-Y529E mutantDepositorInsertHuman AGO2 (AGO2 Human)
UseTagsExpressionMammalianMutationAgo2-Y529EPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe hygro 3xFLAG Dna2 wt
Plasmid#31955DepositorInserthDna2 (DNA2 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationChanged start ATG to AAA,PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIRES-H3.3-WT-FLAG-BFP
Plasmid#206139PurposeExpression of a FLAG tagged human histone H3.3 gene with BFPDepositorInsertH3.3 (H3-3A Human)
UseTagsFLAG-eBFPExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MCS-TurboID-3xFLAG
Plasmid#186814PurposeCAG promoter-driven expression of BioID2-fused protein (C terminus)DepositorTypeEmpty backboneUseTagsTurboID-3xFLAGExpressionMammalianMutationPromoterCAG promoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG
Plasmid#140018PurposePlasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertEcoTyrRS
UseTagsFLAGExpressionMammalianMutationY37L, D182S, F183M, L186APromoterEF1Available sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-GFP-OSBP
Plasmid#134659PurposeFlag and GFP-tagged OSBPDepositorInsertOSBP (OSBP Human)
UseLentiviralTagsFLAG and GFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only