Showing: 2541 - 2560 of 21747 results
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Plasmid#172314PurposeExpresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)DepositorInsertLambda N peptide - human ADAR2 Deaminase Domain E488Q
UseTags6xHis, Flag, and Nuclear Localization Signal of S…ExpressionMammalianMutationchanged the Glutamic acid 488 in the Deaminase Do…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pHOT-P2Y2R
Plasmid#159108Purposeentiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the GibsonDepositorInsertP2Y2R
UseLentiviralTagsExpressionMutationPromoterUBCAvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH
Plasmid#182425PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteinsDepositorInsertsUseTagsVesicle Nucleating peptide (VNp) and Vesicle Nucl…ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 CDS A. thaliana PARP2
Plasmid#175810PurposepENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 2 (PARP2, AT4G02390.1) without stop codon for C-terminal epitope tagsDepositorInsertPARP2 (PARP2 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnoneExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pEMB52/Turkey_34_374
Plasmid#40545DepositorInsertLRRK2
UseBaculovirusTags6xHisExpressionMutationWTPromoterpolHAvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-iLOVf
Plasmid#63723PurposeE coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteinsDepositorTypeEmpty backboneUseTagsTEE, iLOV, 6xHis, thrombin cleavage siteExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP prescission LIC cloning vector (HMPKS)
Plasmid#29721DepositorTypeEmpty backboneUseTagsHis6-MBP-prescissionExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
C95m
Plasmid#121001PurposeMoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.DepositorInsertT7 RNA polymerase (Andy please check!)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
C113m
Plasmid#121012PurposeMoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.DepositorInsertCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
371-A
Plasmid#97026PurposeyORFDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-aASIC1
Plasmid#32795DepositorInsertASIC1
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
mTagBFP-Nucleus-7
Plasmid#55265PurposeLocalization: Nuclear Localization, Excitation: 399, Emission: 456DepositorInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
UseTagsmTagBFP-NLSExpressionMammalianMutationPromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pTRIPZ (M)-HT-NLS
Plasmid#82518PurposeLentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.DepositorInsertNuclear localizaition sequence
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
371-U
Plasmid#97036PurposeMYFQSNA-yORFDepositorTypeEmpty backboneUseTagsMYFQSNAExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1012 - pAAV EF1a DIO Nuc-eYFP
Plasmid#75082PurposeAn AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.DepositorInsertNuc-eYFP
UseAAV and Cre/LoxTagsNLSExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pETDuet_huCAD_ICADL (TevS)
Plasmid#100098Purposedual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequencesDepositorUseTagsExpressionBacterialMutationtwo caspase cleavage sites (DETD-117 and DAVD-224…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET11a_3xFlag-nsp14 (SARS-CoV-2)
Plasmid#169159PurposeTo express SARS-CoV-2 nsp14 in E. coliDepositorInsert3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
UseTags3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…ExpressionBacterialMutationCodon optimised for E. coliPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
COVID-SARS2 NSP14/10
Plasmid#159613PurposeBacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10DepositorInsertsUseTagsHis6, TEV cleavage site and noneExpressionBacterialMutationCodon-optimized for E. coli expressionPromoter(Bicistronic) and T7 - LacOAvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-eGFP-shAqp4-1
Plasmid#223223Purposemir30 based shRNA strategy, shRNA targeting mouse Aqp4DepositorInsertshAqp4 (Aqp4 Mouse)
UseAAVTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-eGFP-shAqp4-3
Plasmid#223224Purposemir30 based shRNA strategy, shRNA targeting mouse Aqp4DepositorInsertshAqp4 (Aqp4 Mouse)
UseAAVTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 2541 - 2560 of 21747 results