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Addgene
Showing: 2521 - 2540 of 3246 results

  1. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

    Plasmid
    #216153
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11'].
    Depositor
    Pietro Fratta
    Article
    Wilkins et al bioRxiv. 2023 Nov 15:2023.11.15.565967.
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216153-plasmid-map-sequence-id-437757

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  2. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

    Plasmid
    #216733
    Purpose
    Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase and sgCTG
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    U6 and CAG
    Availability
    Academic Institutions and Nonprofits only
    216733-plasmid-map-sequence-id-430318

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  3. pX551-miniCMV-SpCas9D10A nickase

    Plasmid
    #216735
    Purpose
    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    T7 and mini-CMV
    Availability
    Academic Institutions and Nonprofits only
    216735-plasmid-map-sequence-id-430321

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  4. LacI-pLacI-pI pm

    Plasmid
    #218098
    Purpose
    Phagemid that expresses LacI, and pI under the Lac promoter
    Depositor
    Kevin Esvelt
    Insert
    pI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac promoter
    Availability
    Academic Institutions and Nonprofits only
    218098-plasmid-map-sequence-id-432462

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  5. LacI-pLacI-pII pm

    Plasmid
    #218099
    Purpose
    Phagemid that expresses LacI, and pII under the Lac promoter
    Depositor
    Kevin Esvelt
    Insert
    pII
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac promoter
    Availability
    Academic Institutions and Nonprofits only
    218099-plasmid-map-sequence-id-432443

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  6. LacI-pLacI-pIII pm

    Plasmid
    #218100
    Purpose
    Phagemid that expresses LacI, and pIII under the Lac promoter
    Depositor
    Kevin Esvelt
    Insert
    pIII
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac promoter
    Availability
    Academic Institutions and Nonprofits only
    218100-plasmid-map-sequence-id-432442

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  7. LacI-pLacI-pIV pm

    Plasmid
    #218101
    Purpose
    Phagemid that expresses LacI, and pIV under the Lac promoter
    Depositor
    Kevin Esvelt
    Insert
    pIV
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac promoter
    Availability
    Academic Institutions and Nonprofits only
    218101-plasmid-map-sequence-id-432440

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  11. pCEE

    Plasmid
    #159746
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al BioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    159746-plasmid-map-sequence-id-313813

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  12. pTEE

    Plasmid
    #159748
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al BioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    159748-plasmid-map-sequence-id-313816

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  13. pYEE

    Plasmid
    #159750
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al BioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    159750-plasmid-map-sequence-id-313819

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Showing: 2521 - 2540 of 3246 results