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Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
UseTags8 histidine tagExpressionBacterialMutationPromoterT7 promotorAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet-15b-ultraID
Plasmid#172879Purposeexpresses ultraID in E. coliDepositorInsertultraID
UseTagsmycExpressionBacterialMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP
Plasmid#164038PurposeExpression of dCas9-14xGFP11 in mammalian cellsDepositorInsertdCas9-14xGFP11
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and H840A in Cas9PromoterdSV40Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sox2-t2A-GFP
Plasmid#127537PurposeExpresses human SOX2 and eGFP via t2A linker.DepositorInsertSOX2 (SOX2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)
Plasmid#225756PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-DIO-EGFP (AAV8)
Viral Prep#50457-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PDGFRA
Plasmid#116769PurposeLentiviral expression of PDGFRADepositorInsertPDGFRA (PDGFRA Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe4_ChR2_mCherry
Plasmid#213937PurposeAAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_ChR2_mCherryDepositorInsertChannelrhodopsin 2-mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP (AAV Retrograde trial size)
Viral Prep#50465-AAVrg.TPurposeReady-to-use AAV Retrograde trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.DepositorPromoterSynTagsEGFPAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG mito-mEos2
Plasmid#168515PurposeExpression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rateDepositorInsertmito-mEos2
UseTagsmEos2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21b(+)-Is-PETase
Plasmid#112202PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
UseTags6XHISExpressionBacterialMutationPromoterN/AAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mitochondrial PancACe
Plasmid#215710PurposeExpresses acetyl-CoA biosensor in the mitochondriaDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and mitochondrial localization sequence…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RPS6 (pDD1902)
Plasmid#52913Purposecontains RPS6 with HA-tag, which can be expressed in mammalian cells. Insert sites are at HindIII and BamHI.DepositorInsertHAS6 (RPS6 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Myoferlin HA
Plasmid#22443DepositorInsertMyoferlin
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8m-WPRE (AAV9)
Viral Prep#179255-AAV9PurposeReady-to-use AAV9 particles produced from AAV-CAG-jGCaMP8m-WPRE (#179255). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8m-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
N174-MCS (Puro)
Plasmid#81068PurposeLentiviral vector for constitutive gene expression.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP1a-EGFP
Plasmid#205303PurposeExpression of WHaloCaMP1a-EGFP fusion in E. coliDepositorInsertWHaloCaMP1a-EGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-DTAG-CTERM-GFP
Plasmid#185765PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterSFFVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only