Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 2521 - 2540 of 147404 results

  8. pFCK_smFP FLAG _ Bright

    Plasmid
    #71815
    Purpose
    Fluorescent version of smFP FLAG
    Depositor
    Loren Looger
    Insert
    smFP FLAG _ Bright
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    71815-plasmid-map-sequence-id-242755

    Enlarge

  9. pCAGmRuby2smFPMyc

    Plasmid
    #71816
    Purpose
    encodes multiple copies of an epitope tag Myc ( not detected by anti GFP ab)
    Depositor
    Loren Looger
    Insert
    mRuby2_smFP_Myc
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    71816-plasmid-map-sequence-id-422684

    Enlarge

  10. pCAGmRuby2smFPV5

    Plasmid
    #71817
    Purpose
    encodes multiple copies of an epitope tag V5( not detected by anti GFP ab)
    Depositor
    Loren Looger
    Insert
    mRuby2_smFP_V5
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only

  17. pET28-Dnmt3a3L-sc27

    Plasmid
    #71827
    Purpose
    For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.
    Depositor
    Albert Jeltsch
    Article
    Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do
    Insert
    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    71827-plasmid-map-sequence-id-254300

    Enlarge

  18. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid
    #71828
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    71828-plasmid-map-sequence-id-131023

    Enlarge

  19. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

    Plasmid
    #71829
    Purpose
    Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    71829-plasmid-map-sequence-id-131024

    Enlarge

Showing: 2521 - 2540 of 147404 results