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Plasmid#212020PurposeFor filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertUtrCH
UseTagsmStayGold(c4)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7s-WPRE (AAV PHP.eB)
Viral Prep#104487-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Grx1-roCherry
Plasmid#141068PurposeFluorescent biosensor for 2GSH/GSSG ratioDepositorInsertGrx1-roCherry
UseTagsExpressionMammalianMutationChanged Alanine 150 to Cysteine, inserted Threon…PromoterCMV, SP6Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
SFFV-IKZF3-CTERM-GFP
Plasmid#185769PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, IKZF3 minidegronExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6d-V106W
Plasmid#215827PurposeExpress CBE6d V106W (with SpCas9) in mammalian cellsDepositorInsertCBE6d V106W-SpCas9-2xUGI (cas9 )
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
UseTagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-WT human MyoD-T2A-dsRedExpress2
Plasmid#60628PurposeExpresses WT human MyoD and dsRedExpress2 in response to doxycyclineDepositorInsertVP64 mouse MyoD (MYOD1 Human)
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcDNA3
Plasmid#18964DepositorInsertFirefly Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-sec61b
Plasmid#186959PurposeExpress sec61b connected with N-terminus HaloTagDepositorInsertSec61 (SEC61B Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-ER GFP
Plasmid#80069PurposeTo express and target GFP into the endoplasmic reticulum using lentivirusDepositorInsertER GFP (SEC61B Human)
UseLentiviralTagsGFP derived from Aequorea coerulescens on the NH2…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_MicroID2-HA
Plasmid#188521PurposeEncodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cellsDepositorInsertMicroID2
UseTransposon mediated gene integrationTagsHAExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
SFFV-HALO-CTERM-GFP
Plasmid#185767PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, HaloExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(ER-mito)
Plasmid#214423PurposeDetection of ER-mito contact siteDepositorInsertRspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito
UseLentiviralTagsCFAST10-Halo and RspAN-FRB-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL1-pCMV7.1
Plasmid#155019PurposeN-terminal 3xFLAG-tagged CUL1 in a pCMV7.1 vectorDepositorInsertCullin 1 (CUL1 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato (AAV1)
Viral Prep#51082-AAV1PurposeReady-to-use AAV1 particles produced from AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato (#51082). In addition to the viral particles, you will also receive purified AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FlpO-bGHpA (AAV Retrograde)
Viral Prep#51669-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV phSyn1(S)-FlpO-bGHpA (#51669). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-FlpO-bGHpA plasmid DNA. hSyn-driven FlpO expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
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MAC_N_IRF1
Plasmid#167760PurposeExpresses IRF1 in mammalian cellsDepositorInsertIRF1 (IRF1 Human)
UseTagsMACExpressionMammalianMutationno mutationsPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro
Plasmid#205631PurposeExpression of TnpBmax in mammalian cellsDepositorInsertISDra2-TnpB optimized for mammalian expression
UseTagsNLS-3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only