Addgene

We narrowed to 30,517 results for: CMV

Showing: 2461 - 2480 of 30517 results

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pCMV-Frankenbody-FLAG-Halo

Plasmid

#175296

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with HaloTag

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-ABEmax-BlastR

Plasmid

#152989

Purpose

A-to-G base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

ABE7.10-nSpCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-ABEmaxCP1041-BlastR

Plasmid

#152990

Purpose

A-to-G base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

ABE7.10-nSpCas9 CP1041

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-AIDmax-BlastR

Plasmid

#152994

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

AID-nSpCas9-UGI-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-BE4maxCP1028-BlastR

Plasmid

#152993

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

BE4-nSpCas9 CP1028-UGI-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-CDAmax-BlastR

Plasmid

#152995

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

CDA-nSpCas9-UGI-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-BSD

Plasmid

#224122

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-BSD

Use

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRNA-CMV-GFP

Plasmid

#85451

Purpose

Express sgRNA in mammalian cells

Depositor

Hetian Lei

Article

Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

Insert

pCMV-EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

pCMV-EGFP

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pJEP207-AAV-CMV-MCS-pA

Plasmid

#78318

Purpose

AAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Cytomegalo Virus(CMV)

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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Stat3-C Flag pRc/CMV

Plasmid

#8722

Depositor

Jim Darnell

Article

Bromberg et al Cell 1999 Aug 6;98(3):295-303.

Insert

Stat3-C (Stat3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

A662C and N664C. Residues changed to Cys renders…

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pSLQ8001 - pCMV-R8.91 (D64V)

Plasmid

#202687

Purpose

R8.91 plasmid with mutated integrase (D64V)

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

gag pol

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

D64V

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-pro-Il1b-C-Flag

Plasmid

#75131

Purpose

Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

interleukin 1 beta (Il1b Mouse)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

(please see depositor comments below)

Promoter

CMV

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2w/b

Plasmid

#111570

Purpose

Expresses a plasma membrane calcium pump hPMCA2w/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

Plasmid

#224123

Purpose

Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-mutant (Q20A_Y23A_Y24F_F35A)

Use

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Q20A_Y23A_Y24F_F35A in La(SSB)1-194

Promoter

CMV

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-GFP-Vpr-RR

Plasmid

#83374

Purpose

Expression of Myc tagged GFP-Vpr fusion protein

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

Myc-GFP-Vpr

Use

Tags

Myc and Myc-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN beta

Plasmid

#40929

Depositor

Scott Maul, Elizabeth Luna

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN beta (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-mCherry-53BP1

Plasmid

#127658

Purpose

Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN alpha

Plasmid

#40928

Depositor

Scott Maul, Elizabeth Luna

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-mCherry-MHC-IIA

Plasmid

#35687

Depositor

Venkaiah Betapudi

Article

Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. E

Insert

MHC IIA (MYH9 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-eBE-Y130F

Plasmid

#113423

Purpose

Expresses hA3A-eBE-Y130F in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-eBE-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

hAPOBEC3A_Y130F

Promoter

CMV

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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