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Showing: 2441 - 2460 of 147404 results

  2. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

    Enlarge

  3. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    71667-plasmid-map-sequence-id-243369

    Enlarge

  4. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid
    #71684
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    71684-plasmid-map-sequence-id-129672

    Enlarge

  5. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    71685-plasmid-map-sequence-id-322543

    Enlarge

  6. pcDNA3.1+ SMN1 myc HIS

    Plasmid
    #71687
    Purpose
    Mammalian expression of SMN1
    Depositor
    Gideon Dreyfuss
    Insert
    SMN1 (SMN1 Human)
    Use
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    71687-plasmid-map-sequence-id-287410

    Enlarge

  7. pJK563

    Plasmid
    #71689
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant (AaPurE1fwf-Y154I)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71689-plasmid-map-sequence-id-131681

    Enlarge

  9. pJK699

    Plasmid
    #71691
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Asp371>Asn mutant (AarAH6-D371N)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed Asp371 to Asn
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    71691-plasmid-map-sequence-id-129671

    Enlarge

  10. pJK708

    Plasmid
    #71692
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp400>Phe mutant (AarAH6-W400F)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed Trp400 to Phe
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    71692-plasmid-map-sequence-id-129670

    Enlarge

  11. pJK241

    Plasmid
    #71693
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp404>Phe mutant (AarAH6-W404F)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed Tro404 to Phe
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    71693-plasmid-map-sequence-id-129669

    Enlarge

  12. pJK580

    Plasmid
    #71694
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag (AaTrxAH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71694-plasmid-map-sequence-id-134489

    Enlarge

  13. pJK581

    Plasmid
    #71695
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag and Cys35>Ser mutant (AaTrxAH6-C35S)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes cysteine-35 to serine
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71695-plasmid-map-sequence-id-134492

    Enlarge

  14. pJK587

    Plasmid
    #71696
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag and Cys138>Ser mutant (AaTrxB1H6-C138S)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase 1
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes cysteine-138 to serine
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71696-plasmid-map-sequence-id-131733

    Enlarge

  15. pJK590

    Plasmid
    #71698
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag (AaTrxB1H6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase 1
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71698-plasmid-map-sequence-id-131731

    Enlarge

  16. pJK615

    Plasmid
    #71699
    Purpose
    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71699-plasmid-map-sequence-id-137034

    Enlarge

  17. pJK645

    Plasmid
    #71700
    Purpose
    Produces E. coli thioredoxin (EcTrxA)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71700-plasmid-map-sequence-id-131737

    Enlarge

  18. pJK647

    Plasmid
    #71701
    Purpose
    Produces E. coli thioredoxin reductase (EcTrxB)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71701-plasmid-map-sequence-id-131729

    Enlarge

  19. pJK651

    Plasmid
    #71702
    Purpose
    Produces E. coli thioredoxin reductase with a C-terminal His6 tag (EcTrxBH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71702-plasmid-map-sequence-id-131727

    Enlarge

  20. pJK394

    Plasmid
    #71703
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin reductase 1 (AaTrxB1)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase 1
    Use
    Tags
    Expression
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71703-plasmid-map-sequence-id-131723

    Enlarge

Showing: 2441 - 2460 of 147404 results