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RPS18_pLX307

Plasmid

#98370

Purpose

Lentiviral expression of RPS18

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

RPS18 (RPS18 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Tsg101

Plasmid

#21505

Depositor

James Hurley

Article

Lee et al Science. 2008 Oct 24. 322(5901):576-80.

Insert

Tsg101 (TSG101 Human)

Use

Tags

Cherry*

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only

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mito-dendra2

Plasmid

#55796

Purpose

photo-activatable mitochondrial marker for expression in mammalian cells

Depositor

David Chan

Article

Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

Insert

mito-Dendra2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Tfap2a

Plasmid

#70274

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2a

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Tfap2a (Tfap2a Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
pGL3 Promoter Vector

Plasmid

#212939

Purpose

Control vector with constitutive firefly luciferase expression

Depositor

Debrya Groskreutz

Insert

luc+

Use

Luciferase

Tags

Expression

Mutation

WT

Promoter

SV40

Available since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEvol-pAzFRS.1.t1

Plasmid

#73547

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.1.t1
pAzFRS.1.t1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-Vimentin-7

Plasmid

#56439

Purpose

Localization: Intermediate Filaments, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

Vimentin (VIM Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pNOC-ARS-CRISPR-v2

Plasmid

#99863

Purpose

Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

Expression

Mutation

Promoter

Ribi

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.PI.EGFP.WPRE.bGH (AAV8)

Viral Prep

#105530-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.

Depositor

James M. Wilson

Promoter

CMV

Tags

EGFP

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only
pFp10

Plasmid

#170460

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

none

Use

Tags

Expression

Insect

Mutation

Promoter

dHsp70

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJH-MBP-CbAgo

Plasmid

#127707

Purpose

pET His6 MBP TEV CbAgo

Depositor

John van der Oost

Article

Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

Insert

CbAgo

Use

Tags

MPB

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

Plasmid

#207664

Purpose

Neuropeptide degradation

Depositor

Sung Han

Article

Kim et al bioRxiv 2023.01.19.524797

Insert

NEP (MME Human)

Use

AAV and Cre/Lox

Tags

mRUBY3

Expression

Mutation

Addition of signaling peptide (POMC) on the N-ter…

Promoter

hSynapsin

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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PLJR965

Plasmid

#115163

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pB80-hKif5b(1-560)rigor-2xmNeongreen

Plasmid

#174649

Purpose

Live-cell marker for stable microtubules

Depositor

Lukas Kapitein

Article

Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

Insert

hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)

Use

Tags

mNeongreen-mNeongreen

Expression

Mammalian

Mutation

rigor mutation G234A

Promoter

Chicken beta-actin

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSMPP-mCherry-mTrim21

Plasmid

#104971

Purpose

Lentiviral Transfer Plasmid with mCherry-tagged mouse Trim21

Depositor

Leo James

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (Trim21 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

SFFV

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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GST-MNase

Plasmid

#136291

Purpose

Expresses Micrococcal nuclease

Depositor

Kavitha Sarma

Article

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

MNase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hHSF1 K80Q

Plasmid

#118351

Purpose

Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

Depositor

Lea Sistonen

Article

Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K80Q

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/hChR2(H134R)-EYFP

Plasmid

#20940

Purpose

Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Tags

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

CMV

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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Cytoplasmic PancACe

Plasmid

#215708

Purpose

Expresses acetyl-CoA biosensor in the cytoplasm

Depositor

Katharine Diehl

Article

Smith et al bioRxiv 2023.12.31.573774

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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