Skip to main content
Addgene
Showing: 2421 - 2440 of 14717 results

  1. pT7CFE1_EVA71_VP12A_C110A

    Plasmid
    #212976
    Purpose
    Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Enterovirus A71 4643 VP12A C110A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    changed cysteine 110 from 2A to alanine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212976-plasmid-map-sequence-id-420360

    Enlarge

  2. pT7CFE1_polio_VP12A_C109R

    Plasmid
    #212987
    Purpose
    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney VP12A C109R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Cysteine 109 from 2A mutated to arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212987-plasmid-map-sequence-id-420359

    Enlarge

  3. pcDNA3-C17orf53_1-87-mCer

    Plasmid
    #211441
    Purpose
    mammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Deletion of aa 88-646
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211441-plasmid-map-sequence-id-419424

    Enlarge

  14. pSBTet(-ΔrtTA)-GB dTomato-P2A-CaMXMT

    Plasmid
    #139176
    Purpose
    SB-transposon with inducible expression of dTomato and Coffea arabica monomethylxathine methyltransferase via P2A self-cleaving linker in a Tet-inducible pSBTRE(-ΔrtTA)-GB backbone.
    Depositor
    Neal Devaraj
    Insert
    CaMXMT
    Use
    Transposon
    Tags
    dTomato-P2A
    Expression
    Mammalian
    Mutation
    P131T on dTomato (Please see depositor comments)
    Promoter
    tight TRE-controlled CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139176-plasmid-map-sequence-id-309363

    Enlarge

Showing: 2421 - 2440 of 14717 results