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Plasmid#13846DepositorInsertJnk1a1(apf) (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationreplaced dual phosporylation motif Thr(183)-Pro-Tā¦PromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJM31
Plasmid#52986PurposeA secreted Annexin V (sAnxV::GFP) Phosphatidylserine sensorDepositorInsertAnnexin V (ANXA5 Human)
UseTagsGFP (w/ introns) and signal sequenceExpressionWormMutationPromoterhsp-16.41Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-LOXCATmut
Plasmid#140085PurposeA control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used originalā¦PromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C-p50
Plasmid#180238PurposeExpresses p50 in mammalian cellsDepositorInsertp50 (DCTN2 Human)
UseTagspmCherry-C1ExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry KNL1 wt hardened
Plasmid#45224DepositorInsertmCherry KNL1 resistant to KNL1 siRNA (KNL1 Human)
UseTagsTEV S15ExpressionMammalianMutationPromoterAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV-YE1-BE3-FNLS-CMV-mCherry
Plasmid#154005PurposeExpress YE1-BE3-FNLSDepositorInsertYE1-BE3-FNLS
UseCRISPRTagsFLAGExpressionMutationPromoterCMVAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGMKOSiE
Plasmid#20865DepositorInsertmyelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
F2RL1-Tango
Plasmid#66277PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertF2RL1 (F2RL1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_sfCherry2(11)_Clathrin light chain
Plasmid#83032PurposeExpresses sfCherry2(11) tagged clathrin light chain in mammalian cellsDepositorInsertsfCherry2(11)_Clathrin light chain (CLTA Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK301
Plasmid#87767PurposeE. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacIDepositorInsertPrhaBAD-sfGFP
UseSynthetic BiologyTags6xHis TagExpressionBacterialMutationPromoterPrhaBAD rhamnose-inducible promoter from E. coliAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
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GAL4-Creb5 (1- 508)
Plasmid#195030PurposeGAL4-Creb5 fusion construct (full length, 508aa)DepositorInsertGAL4-Creb5 fusion construct (full length, 508aa) (CREB5 Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX GFP Mps1 wt
Plasmid#63702PurposeInducible expression of GFP-Mps1 fusion in mammalian cellsDepositorInsertMps1 (TTK Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromotertight TRE promoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)
Plasmid#140892PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.DepositorUseTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE)PromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSI2-DCD
Plasmid#166968PurposeExpress the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADARDepositorInsertMSI2-DCD
UseRetroviralTagsMSI2-DCDExpressionMammalianMutationN495SPromoterMSCVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CLTC_Clathrin propeller
Plasmid#178471PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCLTC_Clathrin propeller (CLTC Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-Nix
Plasmid#100770PurposeLentiviral expression of shRNA targeting NixDepositorInsertLenti-sh-Nix
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBdest-GST-PI3Kc1
Plasmid#187992PurposePlasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326DepositorUseTagsGSTExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLpA-DN.JNK1
Plasmid#51942PurposeAdenoviral vector for dominant negative JNK1 expressionDepositorInsertDN JNK1 (Mapk8 Mouse)
UseAdenoviralTagsFLAGExpressionMutationdominant negative: the dual phosphorylation motiā¦PromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-CMYC T58A
Plasmid#53178PurposeExpresses mouse CMYC T58A from puromycin resistance retroviral vectorDepositorInsertCMYC (Myc Mouse)
UseRetroviralTagsExpressionMammalianMutationT58APromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only