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Plasmid#211895PurposeVinculin conformation sensor (VinCS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.DepositorInsertVinculinCS-S1033D (VCL Chicken, Synthetic)
UseLentiviralTagsExpressionMutationmutated vinculin serine 1033 to aspartic acid (S1…PromoterCMVAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM002-sgRNA-lenti-ZeoR
Plasmid#200060PurposeLentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sitesDepositorInsertBleomycin resistance, mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-CBP(1-287)
Plasmid#182839PurposeCBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)DepositorInsertCBP1-287
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-Elo3-GFP11-Ura
Plasmid#177734PurposeER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA markerDepositorInsertElo3 (ELO3 Budding Yeast)
UseTagsGFP11 and HDELExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-DM-HA-GFP11-KDEL
Plasmid#177723PurposeDM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFlucDM
UseTagsGFP11, HA, KDEL, and Prolactin Signal SequenceExpressionBacterial and MammalianMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP- Ura
Plasmid#177732PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA markerDepositorInsertFlucDM
UseTagsHA, HDEL, KAR2 Signal Sequence, and eGFPExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5E-161K enhancer-NR2F2-MUT
Plasmid#138371PurposeGateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutatedDepositorInsert161k human enhancer with conserved NR2F2 binding sites mutated
UseGateway compatibleTagsExpressionMutationNR2F2 sites mutatedPromoternoneAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-161K enhancer-ETS-CORE-MUT
Plasmid#138372PurposeGateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutatedDepositorInserthuman 161K human enhancer with ETS bind sites mutated
UseGateway compatibleTagsExpressionMutationETS factor binding sites mutatedPromoterNONEAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H
Plasmid#108378PurposeFRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-TPI_WT-xrRNA-4H
Plasmid#108377PurposeFRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mCherry Gamma 3
Plasmid#65234PurposeTo study the role of the differential rates of Gβγ translocation in modulating signaling dynamics in a cellDepositorInsertsUseTags6-His tagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a D540K
Plasmid#53057PurposeExpresses alpha subunit of D540K hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionTagsExpressionMutationchanged Aspartate 540 to LysinePromoterSP6Available sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(C631S, C643S and C699T)
Plasmid#52128Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(M1-5) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationC631S, C643S and C699TPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 T7 Jip1b(471-707)
Plasmid#52428Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(471-707) ) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-470PromoterAvailable sinceApril 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(282-707)
Plasmid#52126Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(282-707)) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-281PromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
Bison sgRNA library
Pooled Library#169942PurposeThe BISON CRISPR library targets 713 E1, E2, and E3 ubiquitin ligases, deubiquitinases, and control genes and contains 2,852 guide RNAs. It was cloned into the pXPR003.DepositorUseLentiviralAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSI-Check2-hRluc-NFkB-firefly
Plasmid#106979PurposeLuciferase reporter with internal control for NFkB signal.DepositorInsertNFKB recognition sites (NFKB1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUS2-SH231
Plasmid#115150PurposeCas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4DepositorInsertDual SH231 gRNAs
UseCRISPRTags3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLSExpressionMammalianMutationPromoterU6, CBhAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-pA-M.EcoGII
Plasmid#192873PurposepA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578DepositorInsertpA-M.EcoGII
UseTags3X Flag TagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZL Blast Snail ER
Plasmid#18798DepositorInsertsnail (SNAI1 Human)
UseRetroviralTagsER hormone binding domainExpressionMammalianMutationG525R mutation in the ER. The presence of the G52…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only