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Plasmid#40270DepositorInsertPTPN1 (PTPN1 Human)
UseTagsmCherryExpressionMammalianMutationD181APromoterCMVAvailable sinceNov. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET_RTX
Plasmid#102787PurposeExpresses the Reverse Transcriptase geneDepositorInsertreverse transcription xenopolymerase
UseTagsExpressionBacterialMutationPromoterT7 promoter + lac operatorAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET_RTX_(exo-)
Plasmid#102786PurposeExpresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)DepositorInsertreverse transcription xenopolymerase with 3'-5' deficient exonuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-LacZ20
Plasmid#65027PurposeGmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 2. See Depositor Comments below for information regarding the lacZ sequence.DepositorTypeEmpty backboneUseTagslacZExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pICSL12006
Plasmid#50270DepositorInsertpromoter + 5'UTR (Cassava Vein Mosaic Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLNC Wnt-1HA
Plasmid#18027DepositorInsertWnt-1 (Wnt1 Mouse)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEERM6 Km
Plasmid#64027PurposePlasmid for integration into sqs site of Synechocystis 6803DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPnrsBAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYEXC3GH
Plasmid#49027PurposeFX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), GFP, and dec…ExpressionYeastMutationPromoterGAL1Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hisx6-Sirt6-H133Y
Plasmid#20279DepositorInsertSirt6 (Sirt6 Mouse)
UseTagsHisx6ExpressionBacterialMutationHistidine 133 to TyrosinePromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hisx6-Sirt6-S56A
Plasmid#20278DepositorInsertSirt6 (Sirt6 Mouse)
UseTagsHisx6ExpressionBacterialMutationSerine 56 to AlaninePromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMA_Auxiliary 1
Plasmid#102709PurposeProvides the Middle-to-End linker 1 which assists Level 2 cloning in Mobius AssemblyDepositorInsertLinker sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Auxiliary 2
Plasmid#102710PurposeProvides the Middle-to-End linker 2 which assists Level 2 cloning in Mobius AssemblyDepositorInsertLinker sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Auxiliary 3
Plasmid#102711PurposeProvides the Middle-to-End linker 3 which assists Level 2 cloning in Mobius AssemblyDepositorInsertLinker sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Auxiliary 4A
Plasmid#102712PurposeProvides the End-to-End linker 4A which assists Level 2 cloning in Mobius AssemblyDepositorInsertLinker sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Auxiliary 4Γ
Plasmid#102714PurposeProvides the End-to-End linker 4Γ which assists Level 2 cloning in Mobius AssemblyDepositorInsertLinker sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Auxiliary 4Δ
Plasmid#102715PurposeProvides the End-to-End linker 4Δ which assists Level 2 cloning in Mobius AssemblyDepositorInsertLinker sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACYC.Taq.1Amb
Plasmid#102794PurposeExpresses Taq DNA polymerases with a premature stop codonDepositorInsertTaq DNA polymerase
UseTagsExpressionBacterialMutationW168Stop; *(see below)PromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBPGUw-HACK-G4>QF2
Plasmid#80277PurposeGAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.DepositorInsertQF2
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
SaMscL (CDelta26)
Plasmid#79027PurposeStaphylococcus aureus MscL with C Terminal Truncation of 26 amino acidsDepositorInsertMscL
UseTags6x Histidine TagExpressionMutationStop codon placed before 26 amino acids at C Term…PromoterT7 PromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only