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Plasmid#20249DepositorInsertubiquitin C (UBC Human)
UseTagsmRFPExpressionMammalianMutationchanged Stop codon 686 to GlycinePromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
RHPMP132 (mEOS3.1-beta Actin)
Plasmid#88836PurposeLocalization by Super-Resolution microscopy of beta-Actin fused to mEOS3.1DepositorInsertbeta-Actin (ACTB Human)
UseTagsmEOS3.1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFT1.0
Plasmid#46138DepositorInsertmCherry
UseCre/Lox; Mutagenic gene trap for zebrafishTagsExpressionMutationPromoterAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBXNAC3GH
Plasmid#47073PurposeFX cloning E.coli expression vector with araBAD promoter, N-terminal Avi-tag, C-terminal 3C protease cleavage site, GFP and 10x His tagsDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), Avi-tag, GFP…ExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBPGUw-HACK-G4>QF2
Plasmid#80277PurposeGAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.DepositorInsertQF2
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICXC3GH
Plasmid#49023PurposeFX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), GFP, and dec…ExpressionYeastMutationPromoterAOX1Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXL-CAG-ICeuI-sHRPaNRX3beta
Plasmid#79910PurposepiggyBac transposon vector, sHRPaNRX3betaDepositorInsertsHRPaNRX3beta
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREXC3H
Plasmid#47078PurposeFX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site and 10x His tagsDepositorTypeEmpty backboneUseLactococcus lactis expressionTags3C protease site (PreScission site) and decaHis (…ExpressionMutationPromoterNIS (nisin)Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCPP5372
Plasmid#128733PurposeGateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal HA tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
GESTALT_pX330-v1
Plasmid#103061Purposeplasmid px330 with guide targeting GESTALT v1 through V5 constructsDepositorInsertintegration of Cas9 with guide targeting GESTALT barcodes V1 to V5
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpAGO207-1251
Plasmid#117854PurposeExpressed KpAGO 207-1251 in E. coli under IPTG-inducible promotorDepositorInsertArgonaute
UseTags6xHis-SUMO encoded in vectorExpressionBacterialMutationmissing first 206 amino acid residuesPromoterT7Available sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3A
Plasmid#47075PurposeFX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His, 3C protease cleavage site and Avi-tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), Avi-tag, and…ExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pERF-donor
Plasmid#112299PurposeCRISPR donor plasmid to tag human transcription factor ERF with GFPDepositorInsertERF homology arms flanking EGFP-IRES-Neo cassette (ERF Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Crispr-V2-HMID.v1
Plasmid#103062PurposeLentiCrisprV2 with guide targeting V1-V5 GESTALT barcodesDepositorInsertCas9 plus expressed GESTALT V1-V5 guide
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pREXNH3
Plasmid#47079PurposeFX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and N-terminal 10x His tag and 3C protease cleavage siteDepositorTypeEmpty backboneUseLactococcus lactis expressionTags3C protease site (PreScission site) and decaHis (…ExpressionMutationPromoterNIS (nisin)Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAndy-NBioTAP
Plasmid#53571PurposePlasmid vector carrying N-Bio-1xproteinA tagDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDXNSM3
Plasmid#49028PurposeFX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), MYC, and str…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFT1.2
Plasmid#46140DepositorInsertmCherry
UseCre/Lox; Mutagenic gene trap for zebrafishTagsExpressionMutationPromoterAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pISH-Pou2f2
Plasmid#84320PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Pou2f2. Not for gene expression.DepositorInsertPou2f2 (Pou2f2 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only