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Showing: 2321 - 2340 of 8045 results

  1. HeFSpCas9

    Plasmid
    #92355
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92355-plasmid-map-sequence-id-197617

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  2. pX330-Flag-dHeFSpCas9

    Plasmid
    #92116
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive HeFSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    Cbh
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92116-plasmid-map-sequence-id-177904

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  14. B52 + SPRTN sgSTOP

    Plasmid
    #100709
    Purpose
    B52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
    Depositor
    Alberto Ciccia
    Article
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Insert
    sgSTOP targeting SPRTN (cloned using BbsI)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoters
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100709-plasmid-map-sequence-id-193207

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  20. B52 + PARP4 sgSTOP

    Plasmid
    #100711
    Purpose
    B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
    Depositor
    Alberto Ciccia
    Article
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Insert
    sgSTOP targeting PARP4 (cloned using BbsI)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoters
    Available since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    100711-plasmid-map-sequence-id-193646

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Showing: 2321 - 2340 of 8045 results