-
Plasmid#193005PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D3)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D3PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D2:Luc
Plasmid#193004PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D2)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D2PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRESS-mScarletI-antiFRET-P2A2-linker_noSacI
Plasmid#191473PurposeNegative control for ratiometric comparison between green fluorescent proteins and sensorsDepositorInsertnone
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRESS-mTq2-antiFRET-P2A2-linker_noSacI
Plasmid#191472PurposeNegative control for ratiometric comparison between red fluorescent proteins and sensorsDepositorInsertnone
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xnls-G-Ca-FLITS
Plasmid#191463PurposeGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime changeDepositorInsertG-Ca-FLITS
UseTags3xNLSExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
MON +T111H
Plasmid#187432PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged threonine at 117 to histidinePromoterPH and p10Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
TEG +H122D
Plasmid#187434PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged histidine at 128 to aspartic acidPromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FER +H111E
Plasmid#187429PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged histidine at 117 to glutamic acidPromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
KEE +E111H
Plasmid#187431PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged glutamic acid at 117 to histidinePromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNG +R111Q
Plasmid#187437PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged arginine at 117 to glutaminePromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FER +H122D
Plasmid#187428PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged histidine at 128 to aspartic acidPromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
MON +N122H
Plasmid#187433PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged asparagine at 128 to histidinePromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
OST +Q111R
Plasmid#187436PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged glutamine at 116 to argininePromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FER +H111T
Plasmid#187430PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged histidine at 117 to threoninePromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
CHI +N122D
Plasmid#187427PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged asparagine at 130 to aspartic acidPromoterPH and p10Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HSPA8-Halo
Plasmid#175325PurposeProduction of HaloTag fusion proteinDepositorInsertHSPA8 (HSPA8 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_xtHUR-Halo
Plasmid#175314PurposeProduction of HaloTag fusion proteinDepositorInsertHUR (elavl1 X. tropicalis (frog))
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB235
Plasmid#170024PurposeEncodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-p65(100-261)-RTA(75-190)
Plasmid#99672PurposeExpresses dSp Cas9 fused to combination of truncated activation domainsDepositorArticleInsertdCas9
UseTagsVP64-p65(101-261)-RTA(75-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTR596Q-myc
Plasmid#107483PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R596Q conferring gain of function phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationY289IPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only