pH6HTC_HSPA8-Halo
(Plasmid
#175325)
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PurposeProduction of HaloTag fusion protein
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175325 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepH6HTC_minus_cer-region
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Vector typeBacterial Expression ; Cell Free Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHSPA8
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SpeciesH. sapiens (human)
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Entrez GeneHSPA8 (a.k.a. HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71)
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Tag
/ Fusion Protein
- HaloTag-6xHis (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.11.08.467743 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pH6HTC_HSPA8-Halo was a gift from Claudia Kutter (Addgene plasmid # 175325 ; http://n2t.net/addgene:175325 ; RRID:Addgene_175325) -
For your References section:
Large-scale identification of RBP-RNA interactions by RAPseq refines essentials of post-transcriptional gene regulation. Atanasoai I, Papavasileiou S, Preiß N, Kutter C. bioRxiv 2021.11.08.467743 10.1101/2021.11.08.467743